Serologic dissection of HLA-D specificities by the use of monoclonal antibodies

Masanori Kasahara, Toshinao Takenouchi, Kazumasa Ogasawara, Hitoshi Ikeda, Tsuguyo Okuyama, Naoshi Ishikawa, Junko Moriuchi, Akemi Wakisaka, Yuko Kikuchi, Miki Aizawa, Takehisa Kaneko, Noboru Kashiwagi, Yasuharu Nishimura, Takehiko Sasazuki

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Abstract

To study the gene products of the HLA complex, we produced two monoclonal antibodies, termed HU-18 and HU-23. They were active in complement-dependent cytotoxicity and detected B-cell alloantigens encoded by a locus (or loci) linked to HLA. When three types of HLA-DR4 homozygous B-cell lines with different HLA-D specificities were tested for reactivity with HU-18 and HU-23, they displayed distinct reaction patterns depending on the HLA-D specificities they possessed: EBV-Wa (HLA-DYT homozygous), negative for both HU-18 and HU-23; KT2 and KOB (HLA-DKT2 homozygous), positive only for HU-18; and ER (HLA-Dw4 homozygous), positive for both. These differential reaction patterns were further confirmed by testing against a panel of 17 HLA-DR4-positive peripheral blood lymphocytes with known HLA-D specificities. Thus, these monoclonal antibodies allow us to identify HLA-DYT, HLA-DKT2, and HLA-Dw4 solely by serologic methods. This is the first clearcut serologic identification of these three HLA-DR4-associated HLA-D specificities, which have been indistinguishable by conventional serology and identified only by cellular techniques. It is hoped that immunochemical investigations using HU-18 and HU-23 will advance our understanding of the HLA-D region on a molecular level.

Original languageEnglish
Pages (from-to)485-495
Number of pages11
JournalImmunogenetics
Volume17
Issue number5
DOIs
Publication statusPublished - Jan 1 1983
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Kasahara, M., Takenouchi, T., Ogasawara, K., Ikeda, H., Okuyama, T., Ishikawa, N., ... Sasazuki, T. (1983). Serologic dissection of HLA-D specificities by the use of monoclonal antibodies. Immunogenetics, 17(5), 485-495. https://doi.org/10.1007/BF00696872