Serotonin-induced regulation of the actin network for learning-related synaptic growth requires Cdc42, N-WASP, and PAK in Aplysia sensory neurons

Hiroshi Udo, Iksung Jin, Joung Hun Kim, Hsiu Ling Li, Trisha Youn, Robert D. Hawkins, Eric R. Kandel, Craig H. Bailey

Research output: Contribution to journalArticle

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Abstract

Application of Clostridium difficile toxin B, an inhibitor of the Rho family of GTPases, at the Aplysia sensory to motor neuron synapse blocks long-term facilitation and the associated growth of new sensory neuron varicosities induced by repeated pulses of serotonin (5-HT). We have isolated cDNAs encoding Aplysia Rho, Rac, and Cdc42 and found that Rho and Rac had no effect but that overexpression in sensory neurons of a dominant-negative mutant of ApCdc42 or the CRIB domains of its downstream effectors PAK and N-WASP selectively reduces the long-term changes in synaptic strength and structure. FRET analysis indicates that 5-HT activates ApCdc42 in a subset of varicosities contacting the postsynaptic motor neuron and that this activation is dependent on the PI3K and PLC signaling pathways. The 5-HT-induced activation of ApCdc42 initiates reorganization of the presynaptic actin network leading to the outgrowth of filopodia, some of which are morphological precursors for the learning-related formation of new sensory neuron varicosities.

Original languageEnglish
Pages (from-to)887-901
Number of pages15
JournalNeuron
Volume45
Issue number6
DOIs
Publication statusPublished - Mar 24 2005

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Aplysia
Sensory Receptor Cells
Actins
Serotonin
Learning
Motor Neurons
Growth
rho GTP-Binding Proteins
Pseudopodia
Phosphatidylinositol 3-Kinases
Synapses
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Serotonin-induced regulation of the actin network for learning-related synaptic growth requires Cdc42, N-WASP, and PAK in Aplysia sensory neurons. / Udo, Hiroshi; Jin, Iksung; Kim, Joung Hun; Li, Hsiu Ling; Youn, Trisha; Hawkins, Robert D.; Kandel, Eric R.; Bailey, Craig H.

In: Neuron, Vol. 45, No. 6, 24.03.2005, p. 887-901.

Research output: Contribution to journalArticle

Udo, Hiroshi ; Jin, Iksung ; Kim, Joung Hun ; Li, Hsiu Ling ; Youn, Trisha ; Hawkins, Robert D. ; Kandel, Eric R. ; Bailey, Craig H. / Serotonin-induced regulation of the actin network for learning-related synaptic growth requires Cdc42, N-WASP, and PAK in Aplysia sensory neurons. In: Neuron. 2005 ; Vol. 45, No. 6. pp. 887-901.
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AU - Li, Hsiu Ling

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AU - Hawkins, Robert D.

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