Serotonin-induced regulation of the actin network for learning-related synaptic growth requires Cdc42, N-WASP, and PAK in Aplysia sensory neurons

Hiroshi Udo, Iksung Jin, Joung Hun Kim, Hsiu Ling Li, Trisha Youn, Robert D. Hawkins, Eric R. Kandel, Craig H. Bailey

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Application of Clostridium difficile toxin B, an inhibitor of the Rho family of GTPases, at the Aplysia sensory to motor neuron synapse blocks long-term facilitation and the associated growth of new sensory neuron varicosities induced by repeated pulses of serotonin (5-HT). We have isolated cDNAs encoding Aplysia Rho, Rac, and Cdc42 and found that Rho and Rac had no effect but that overexpression in sensory neurons of a dominant-negative mutant of ApCdc42 or the CRIB domains of its downstream effectors PAK and N-WASP selectively reduces the long-term changes in synaptic strength and structure. FRET analysis indicates that 5-HT activates ApCdc42 in a subset of varicosities contacting the postsynaptic motor neuron and that this activation is dependent on the PI3K and PLC signaling pathways. The 5-HT-induced activation of ApCdc42 initiates reorganization of the presynaptic actin network leading to the outgrowth of filopodia, some of which are morphological precursors for the learning-related formation of new sensory neuron varicosities.

Original languageEnglish
Pages (from-to)887-901
Number of pages15
JournalNeuron
Volume45
Issue number6
DOIs
Publication statusPublished - Mar 24 2005

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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