Serum factors from patients with systemic lupus erythematosus enhancing superoxide generation by normal neutrophils

M. Shingu, M. Oribe, T. Todoroki, K. Tatsukawa, K. Tomo-oka, M. Yasuda, M. Nobunaga

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

It has been suggested that human neutrophils exposed to preformed immune complexes or activated complement fragments generate O2- anions in extracellular medium. In vivo studies have revealed that oxygen intermediates produced by immune complex-activated neutrophils play an important role in subsequent tissue damage. Since it is difficult to obtain direct evidence that O2- is released into plasma in patients with systemic lupus erythematosus (SLE), we studied the capacities of their sera to stimulate O2- release by human neutrophils in vitro. Sera from patients with SLE significantly enhanced O2- generation by neutrophils compared to normal sera. The enhancing activity of serum in the induction of increased O2- generation correlated positively with the presence of serum immune complexes and negatively with serum complement levels. The enhancing factors were analyzed by serum fractionation on Sephadex G-200 gel filtration, and were concluded to be immune complexes of intermediate size containing an activated complement fragment.

Original languageEnglish
Pages (from-to)212-215
Number of pages4
JournalUnknown Journal
Volume81
Issue number3
DOIs
Publication statusPublished - 1983

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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