Serum MicroRNA-150 Predicts Prognosis for Early-Stage Non-Small Cell Lung Cancer and Promotes Tumor Cell Proliferation by Targeting Tumor Suppressor Gene SRCIN1

Liren Zhang, Jing Lin, Yuanqing Ye, Taro Oba, Emanuela Gentile, Jie Lian, Jing Wang, Yang Zhao, Jian Gu, Ignacio I. Wistuba, Jack A. Roth, Lin Ji, Xifeng Wu

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12 Citations (Scopus)

Abstract

This integrative multistage study was aimed to identify circulating microRNAs (miRNAs) as prognostic biomarkers and investigate the treatment target for early-stage non-small cell lung cancer (NSCLC) patients. In stage I–II NSCLC patients, we screened and validated the miRNA ratio signatures predictive of prognosis in serum. In tumor, we found that the expression of miR-150 in identified miRNA signatures was also associated with survival. Increased miR-150 expression promoted NSCLC cell proliferation and migration and vice versa. Specific mRNA cleavage sites targeted by endogenous miR-150 in 3′ untranslated region (UTR) of SRCIN1 was identified by utilizing our recently developed novel Stem-Loop-Array reverse-transcription polymerase chain reaction (SLA-RT-PCR) assay. The blocking action of miR-150 resulted in repressed NSCLC cell growth in vitro and knockdown of miR-150 caused substantial tumor volume reduction in vivo. Our findings suggest that miR-150 binding on specific recognition sites in 3′ UTR of tumor suppressor gene SRCIN1 present a potential therapeutic target for NSCLC.

Original languageEnglish
Pages (from-to)1061-1073
Number of pages13
JournalClinical Pharmacology and Therapeutics
Volume103
Issue number6
DOIs
Publication statusPublished - Jun 2018

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

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    Zhang, L., Lin, J., Ye, Y., Oba, T., Gentile, E., Lian, J., Wang, J., Zhao, Y., Gu, J., Wistuba, I. I., Roth, J. A., Ji, L., & Wu, X. (2018). Serum MicroRNA-150 Predicts Prognosis for Early-Stage Non-Small Cell Lung Cancer and Promotes Tumor Cell Proliferation by Targeting Tumor Suppressor Gene SRCIN1. Clinical Pharmacology and Therapeutics, 103(6), 1061-1073. https://doi.org/10.1002/cpt.870