Serum protein kinase Cα as a diagnostic biomarker of cancers

Jeong Hun Kang, Takeshi Mori, Hirotaro Kitazaki, Takuro Niidome, Koichi Takayama, Yoichi Nakanishi, Yoshiki Katayama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

BACKGROUND: Several serum biomarkers such as antigens, soluble proteins, metabolites, and genes have been identified for the diagnosis of cancers and for monitoring the recurrence after cancer treatment. METHODS: In the present study, a protein kinase C (PKC) α-specific peptide substrate was phosphorylated with serum samples collected from cancer-bearing mice (U87, A431, HepG2, and A549) and the phosphorylation ratio was detected by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). RESULTS: The phosphorylation ratio for peptide substrates significantly increased in the serum of cancer-bearing mice compared with the ratio found in control mice. The addition of a PKCα inhibitor induced a concentration-dependent decrease in phosphorylation ratios, but the non-PKCα inhibitors, rottlerin and H-89, did not significantly effect phosphorylation ratios. CONCLUSIONS: These results suggest that serum activated PKCα is a good biomarker applicable to cancer diagnosis.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalCancer Biomarkers
Volume13
Issue number2
DOIs
Publication statusPublished - 2013

All Science Journal Classification (ASJC) codes

  • Oncology
  • Genetics
  • Cancer Research

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    Kang, J. H., Mori, T., Kitazaki, H., Niidome, T., Takayama, K., Nakanishi, Y., & Katayama, Y. (2013). Serum protein kinase Cα as a diagnostic biomarker of cancers. Cancer Biomarkers, 13(2), 99-103. https://doi.org/10.3233/CBM-130340