Serum starvation induces abnormal spindle location, RhoA delocalization, and extension of intercellular bridge with the midbody

Kazunori Nishimura, Sumiko Watanabe, Tsunehisa Kaku, Setsuo Sugishima

Research output: Contribution to journalArticle

Abstract

Serum starvation induces binucleation in HeLa cells, but the effects of serum starvation on mitosis and the significance of binucleation remain unknown. We investigated the effect of serum starvation on mitosis and analyzed the growth of binucleated cells. The frequency of binucleation caused by cytokinesis failure in DMEM without FBS (0% medium) was higher than that in DMEM with FBS (10% medium). In 0% medium, the metaphase spindle location was off-center, and RhoA localization significantly lacked symmetry. The frequency of the extension of intercellular bridge with the midbody in 0% medium was significantly higher than that in 10% medium. Moreover, all mononucleated mitotic cells caused bipolar mitosis and produced only mononucleated daughter cells, but binucleated cells produced various nucleated cells by multipolar mitosis in 0% medium. These results suggest that serum starvation may have various effects on mitosis, and binucleated cells may be related to formation of aneuploidy.

Original languageEnglish
Pages (from-to)821-826
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number5
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Starvation
Mitosis
Cells
Serum
Cytokinesis
Aneuploidy
Metaphase
HeLa Cells
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Serum starvation induces abnormal spindle location, RhoA delocalization, and extension of intercellular bridge with the midbody. / Nishimura, Kazunori; Watanabe, Sumiko; Kaku, Tsunehisa; Sugishima, Setsuo.

In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 5, 01.01.2018, p. 821-826.

Research output: Contribution to journalArticle

@article{51065d1c99514ffeb8e755c4162fdd8b,
title = "Serum starvation induces abnormal spindle location, RhoA delocalization, and extension of intercellular bridge with the midbody",
abstract = "Serum starvation induces binucleation in HeLa cells, but the effects of serum starvation on mitosis and the significance of binucleation remain unknown. We investigated the effect of serum starvation on mitosis and analyzed the growth of binucleated cells. The frequency of binucleation caused by cytokinesis failure in DMEM without FBS (0{\%} medium) was higher than that in DMEM with FBS (10{\%} medium). In 0{\%} medium, the metaphase spindle location was off-center, and RhoA localization significantly lacked symmetry. The frequency of the extension of intercellular bridge with the midbody in 0{\%} medium was significantly higher than that in 10{\%} medium. Moreover, all mononucleated mitotic cells caused bipolar mitosis and produced only mononucleated daughter cells, but binucleated cells produced various nucleated cells by multipolar mitosis in 0{\%} medium. These results suggest that serum starvation may have various effects on mitosis, and binucleated cells may be related to formation of aneuploidy.",
author = "Kazunori Nishimura and Sumiko Watanabe and Tsunehisa Kaku and Setsuo Sugishima",
year = "2018",
month = "1",
day = "1",
doi = "10.1080/09168451.2018.1443791",
language = "English",
volume = "82",
pages = "821--826",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "5",

}

TY - JOUR

T1 - Serum starvation induces abnormal spindle location, RhoA delocalization, and extension of intercellular bridge with the midbody

AU - Nishimura, Kazunori

AU - Watanabe, Sumiko

AU - Kaku, Tsunehisa

AU - Sugishima, Setsuo

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Serum starvation induces binucleation in HeLa cells, but the effects of serum starvation on mitosis and the significance of binucleation remain unknown. We investigated the effect of serum starvation on mitosis and analyzed the growth of binucleated cells. The frequency of binucleation caused by cytokinesis failure in DMEM without FBS (0% medium) was higher than that in DMEM with FBS (10% medium). In 0% medium, the metaphase spindle location was off-center, and RhoA localization significantly lacked symmetry. The frequency of the extension of intercellular bridge with the midbody in 0% medium was significantly higher than that in 10% medium. Moreover, all mononucleated mitotic cells caused bipolar mitosis and produced only mononucleated daughter cells, but binucleated cells produced various nucleated cells by multipolar mitosis in 0% medium. These results suggest that serum starvation may have various effects on mitosis, and binucleated cells may be related to formation of aneuploidy.

AB - Serum starvation induces binucleation in HeLa cells, but the effects of serum starvation on mitosis and the significance of binucleation remain unknown. We investigated the effect of serum starvation on mitosis and analyzed the growth of binucleated cells. The frequency of binucleation caused by cytokinesis failure in DMEM without FBS (0% medium) was higher than that in DMEM with FBS (10% medium). In 0% medium, the metaphase spindle location was off-center, and RhoA localization significantly lacked symmetry. The frequency of the extension of intercellular bridge with the midbody in 0% medium was significantly higher than that in 10% medium. Moreover, all mononucleated mitotic cells caused bipolar mitosis and produced only mononucleated daughter cells, but binucleated cells produced various nucleated cells by multipolar mitosis in 0% medium. These results suggest that serum starvation may have various effects on mitosis, and binucleated cells may be related to formation of aneuploidy.

UR - http://www.scopus.com/inward/record.url?scp=85046695173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046695173&partnerID=8YFLogxK

U2 - 10.1080/09168451.2018.1443791

DO - 10.1080/09168451.2018.1443791

M3 - Article

VL - 82

SP - 821

EP - 826

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 5

ER -