Sesamin prevents decline in exercise capacity and impairment of skeletal muscle mitochondrial function in mice with high-fat diet-induced diabetes

Shingo Takada, Shintaro Kinugawa, Shoji Matsushima, Daisuke Takemoto, Takaaki Furihata, Wataru Mizushima, Arata Fukushima, Takashi Yokota, Yoshiko Ono, Hiroshi Shibata, Koichi Okita, Hiroyuki Tsutsui

Research output: Contribution to journalArticle

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Abstract

We previously reported that exercise capacity and skeletal muscle mitochondrial function in diabetic mice were impaired, in association with the activation of NAD(P)H oxidase. It has been reported that sesamin inhibits NAD(P)H oxidase-induced superoxide production. Therefore, we examined whether the antioxidant sesamin could prevent a decline in exercise capacity in mice with high-fat diet (HFD)-induced diabetes. C57BL/6J mice were fed a normal diet (ND) or HFD, then treated or not with sesamin (0.2%) to yield the following four groups: ND, ND+Sesamin, HFD and HFD+Sesamin (n = 10 each). After 8 weeks, body weight, fat weight, blood glucose, insulin, triglyceride, total cholesterol and fatty acid were significantly increased in HFD compared with ND mice. Sesamin prevented the increases in blood insulin and lipid levels in HFD-fed mice, but did not affect the plasma glucose. Exercise capacity determined by treadmill tests was significantly reduced in HFD mice, but almost completely recovered in HFD+Sesamin mice. Citrate synthase activity was significantly decreased in the skeletal muscle of HFD mice, and these decreases were also inhibited by sesamin. Superoxide anion and NAD(P)H oxidase activity were significantly increased in HFD mice compared with the ND mice and were ameliorated by sesamin. Sesamin prevented the decline in exercise capacity in HFD-induced diabetic mice via maintenance of mitochondrial function, fat oxidation and attenuation of oxidative stress in the skeletal muscle. Our data suggest that sesamin may be useful as a novel agent for the treatment of diabetes mellitus.

Original languageEnglish
Pages (from-to)1319-1330
Number of pages12
JournalExperimental Physiology
Volume100
Issue number11
DOIs
Publication statusPublished - Nov 1 2015

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High Fat Diet
Skeletal Muscle
NADPH Oxidase
Diet
Superoxides
sesamin
Fats
Insulin
Citrate (si)-Synthase
Exercise Test
Inbred C57BL Mouse
Blood Glucose
Diabetes Mellitus
Triglycerides
Oxidative Stress
Fatty Acids
Antioxidants
Cholesterol
Body Weight
Maintenance

All Science Journal Classification (ASJC) codes

  • Physiology
  • Nutrition and Dietetics
  • Physiology (medical)

Cite this

Sesamin prevents decline in exercise capacity and impairment of skeletal muscle mitochondrial function in mice with high-fat diet-induced diabetes. / Takada, Shingo; Kinugawa, Shintaro; Matsushima, Shoji; Takemoto, Daisuke; Furihata, Takaaki; Mizushima, Wataru; Fukushima, Arata; Yokota, Takashi; Ono, Yoshiko; Shibata, Hiroshi; Okita, Koichi; Tsutsui, Hiroyuki.

In: Experimental Physiology, Vol. 100, No. 11, 01.11.2015, p. 1319-1330.

Research output: Contribution to journalArticle

Takada, S, Kinugawa, S, Matsushima, S, Takemoto, D, Furihata, T, Mizushima, W, Fukushima, A, Yokota, T, Ono, Y, Shibata, H, Okita, K & Tsutsui, H 2015, 'Sesamin prevents decline in exercise capacity and impairment of skeletal muscle mitochondrial function in mice with high-fat diet-induced diabetes', Experimental Physiology, vol. 100, no. 11, pp. 1319-1330. https://doi.org/10.1113/EP085251
Takada, Shingo ; Kinugawa, Shintaro ; Matsushima, Shoji ; Takemoto, Daisuke ; Furihata, Takaaki ; Mizushima, Wataru ; Fukushima, Arata ; Yokota, Takashi ; Ono, Yoshiko ; Shibata, Hiroshi ; Okita, Koichi ; Tsutsui, Hiroyuki. / Sesamin prevents decline in exercise capacity and impairment of skeletal muscle mitochondrial function in mice with high-fat diet-induced diabetes. In: Experimental Physiology. 2015 ; Vol. 100, No. 11. pp. 1319-1330.
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