Several genes expression on fructification of Lentinula edodes

Sang Sun Lee, Shoji Ohga

Research output: Contribution to journalArticle

Abstract

Lentinula edodes was cultivated in the artificial blocks of sawdust, and the fruiting body was induced on the surface of the sawdust blocks. The four different fungal stages; mycelia (D), primordia (C), stipes (B), and pileus (A) of fruiting bodies were collected for the extraction of mRNA, searching the genes related to morphogenesis. The three fragments of cDNA not showed in the stage (A) were picked up and displayed, to be two bands in the agarose gels. Total five bands were selected for analyzing the DNA sequences, using the pRIP-T™ vector system. The fragments of cDNA investigated in this work were not those related to the structural genes (exons), but to be related to the controlling genes (introns).

Original languageEnglish
Pages (from-to)47-59
Number of pages13
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume46
Issue number1
Publication statusPublished - 2001

Fingerprint

Shiitake Mushrooms
Lentinula edodes
sawdust
fruiting bodies
Gene Expression
gene expression
structural genes
Complementary DNA
agarose
Genes
mycelium
morphogenesis
exons
introns
Dilatation and Curettage
genes
Mycelium
gels
Morphogenesis
nucleotide sequences

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Several genes expression on fructification of Lentinula edodes. / Lee, Sang Sun; Ohga, Shoji.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 46, No. 1, 2001, p. 47-59.

Research output: Contribution to journalArticle

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