Several novel method for immobilization of enzymes, microbial cells and organelles

Saburo Fukui, Kenji Sonomoto, Nobuya Itoh, Atsuo Tanaka

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Two novel methods - «photo-crosslinkable resin prepolymer method and «urethane prepolymer method - have been developed in our laboratory. These methods have the following advantages : 1) Prepolymers of desired properties, such as optional chain length, hydrophilicity or hydrophobicity, and ionic character etc., can be used for entrapment of biocatalysts ; (2) preparation of gel-entrapped biocatalysts can be easily achieved under very mild conditions. Photo-crosslinked gels are conveniently obtained by several minutes illumination with near-UV light, of a mixture of liquid prepolymers having photo-sensitive functional groups, an appropriate sensitizer and the solution or suspension of biocatalyst. Formation of polyurethane gels is completed by only mixing water-miscible urethane prepolymers with the aqueous solution or suspension of biocatalyst. The biocatalysts entrapped by these methods are useful for a variety of purposes.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalBiochimie
Volume62
Issue number5-6
DOIs
Publication statusPublished - Jun 10 1980

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All Science Journal Classification (ASJC) codes

  • Biochemistry

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