Several novel method for immobilization of enzymes, microbial cells and organelles

Saburo Fukui, Kenji Sonomoto, Nobuya Itoh, Atsuo Tanaka

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Two novel methods - «photo-crosslinkable resin prepolymer method and «urethane prepolymer method - have been developed in our laboratory. These methods have the following advantages : 1) Prepolymers of desired properties, such as optional chain length, hydrophilicity or hydrophobicity, and ionic character etc., can be used for entrapment of biocatalysts ; (2) preparation of gel-entrapped biocatalysts can be easily achieved under very mild conditions. Photo-crosslinked gels are conveniently obtained by several minutes illumination with near-UV light, of a mixture of liquid prepolymers having photo-sensitive functional groups, an appropriate sensitizer and the solution or suspension of biocatalyst. Formation of polyurethane gels is completed by only mixing water-miscible urethane prepolymers with the aqueous solution or suspension of biocatalyst. The biocatalysts entrapped by these methods are useful for a variety of purposes.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalBiochimie
Volume62
Issue number5-6
DOIs
Publication statusPublished - Jun 10 1980
Externally publishedYes

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Immobilization
Organelles
Enzymes
Gels
Urethane
Hydrophobic and Hydrophilic Interactions
Suspensions
Polyurethanes
Hydrophilicity
Ultraviolet Rays
Hydrophobicity
Lighting
Chain length
Ultraviolet radiation
Functional groups
Resins
Water
Liquids

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Several novel method for immobilization of enzymes, microbial cells and organelles. / Fukui, Saburo; Sonomoto, Kenji; Itoh, Nobuya; Tanaka, Atsuo.

In: Biochimie, Vol. 62, No. 5-6, 10.06.1980, p. 381-386.

Research output: Contribution to journalArticle

Fukui, Saburo ; Sonomoto, Kenji ; Itoh, Nobuya ; Tanaka, Atsuo. / Several novel method for immobilization of enzymes, microbial cells and organelles. In: Biochimie. 1980 ; Vol. 62, No. 5-6. pp. 381-386.
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