Severe focal sialadenitis and dacryoadenitis in NZM2328 mice induced by MCMV

A novel model for human Sjögren's syndrome

Yukiko Ohyama, Virginia A. Carroll, Umesh Deshmukh, Felicia Gaskin, Michael G. Brown, Shu Man Fu

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The genetic and environmental factors that control the development of Sjögren's syndrome, an autoimmune disease mainly involving the salivary and lacrimal glands, are poorly understood. Viruses which infect the glands may act as a trigger for disease. The ability of sialotropic murine CMV (MCMV) to induce acute and chronic glandular disease was characterized in an autoimmune-prone mouse strain, NZM2328. MCMV levels were detectable in the salivary and lacrimal glands 14-28 days after i.p. infection and correlated with acute inflammation in the submandibular gland. After latency, virus was undetectable in the glands by PCR. At this stage, NZM2328 female mice developed severe chronic periductal inflammation in both submandibular and lacrimal glands in contrast to the much milder infiltrates found in female B6-lpr and male NZM2328. The focal infiltrates consisted of CD4+ and B220+ cells as opposed to diffuse CD4+, CD8+, and B220 + cells during acute infection. Salivary gland functional studies revealed a gender-specific progressive loss of secretory function between days 90 and 125 postinfection. Latent MCMV infection did not significantly affect the low incidence of autoantibodies to Ro/SSA and La/SSB Ags in NZM2328 mice. However, reactivities to other salivary and lacrimal gland proteins were readily detected. MCMV infection did not significantly alter the spontaneous onset of kidney disease in NZM2328. Thus, chronic inflammation induced by MCMV with decreased secretory function in NZM2328 mice resembles the disease manifestations of human Sjögren's syndrome.

Original languageEnglish
Pages (from-to)7391-7397
Number of pages7
JournalJournal of Immunology
Volume177
Issue number10
DOIs
Publication statusPublished - Nov 15 2006

Fingerprint

Dacryocystitis
Sialadenitis
Lacrimal Apparatus
Salivary Glands
Submandibular Gland
Infection
Inflammation
Salivary Proteins and Peptides
Viruses
Kidney Diseases
Autoantibodies
Autoimmune Diseases
Chronic Disease
Polymerase Chain Reaction
Incidence

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Severe focal sialadenitis and dacryoadenitis in NZM2328 mice induced by MCMV : A novel model for human Sjögren's syndrome. / Ohyama, Yukiko; Carroll, Virginia A.; Deshmukh, Umesh; Gaskin, Felicia; Brown, Michael G.; Fu, Shu Man.

In: Journal of Immunology, Vol. 177, No. 10, 15.11.2006, p. 7391-7397.

Research output: Contribution to journalArticle

Ohyama, Yukiko ; Carroll, Virginia A. ; Deshmukh, Umesh ; Gaskin, Felicia ; Brown, Michael G. ; Fu, Shu Man. / Severe focal sialadenitis and dacryoadenitis in NZM2328 mice induced by MCMV : A novel model for human Sjögren's syndrome. In: Journal of Immunology. 2006 ; Vol. 177, No. 10. pp. 7391-7397.
@article{fbd332d770b54dc091709d3a9284be8d,
title = "Severe focal sialadenitis and dacryoadenitis in NZM2328 mice induced by MCMV: A novel model for human Sj{\"o}gren's syndrome",
abstract = "The genetic and environmental factors that control the development of Sj{\"o}gren's syndrome, an autoimmune disease mainly involving the salivary and lacrimal glands, are poorly understood. Viruses which infect the glands may act as a trigger for disease. The ability of sialotropic murine CMV (MCMV) to induce acute and chronic glandular disease was characterized in an autoimmune-prone mouse strain, NZM2328. MCMV levels were detectable in the salivary and lacrimal glands 14-28 days after i.p. infection and correlated with acute inflammation in the submandibular gland. After latency, virus was undetectable in the glands by PCR. At this stage, NZM2328 female mice developed severe chronic periductal inflammation in both submandibular and lacrimal glands in contrast to the much milder infiltrates found in female B6-lpr and male NZM2328. The focal infiltrates consisted of CD4+ and B220+ cells as opposed to diffuse CD4+, CD8+, and B220 + cells during acute infection. Salivary gland functional studies revealed a gender-specific progressive loss of secretory function between days 90 and 125 postinfection. Latent MCMV infection did not significantly affect the low incidence of autoantibodies to Ro/SSA and La/SSB Ags in NZM2328 mice. However, reactivities to other salivary and lacrimal gland proteins were readily detected. MCMV infection did not significantly alter the spontaneous onset of kidney disease in NZM2328. Thus, chronic inflammation induced by MCMV with decreased secretory function in NZM2328 mice resembles the disease manifestations of human Sj{\"o}gren's syndrome.",
author = "Yukiko Ohyama and Carroll, {Virginia A.} and Umesh Deshmukh and Felicia Gaskin and Brown, {Michael G.} and Fu, {Shu Man}",
year = "2006",
month = "11",
day = "15",
doi = "10.4049/jimmunol.177.10.7391",
language = "English",
volume = "177",
pages = "7391--7397",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "10",

}

TY - JOUR

T1 - Severe focal sialadenitis and dacryoadenitis in NZM2328 mice induced by MCMV

T2 - A novel model for human Sjögren's syndrome

AU - Ohyama, Yukiko

AU - Carroll, Virginia A.

AU - Deshmukh, Umesh

AU - Gaskin, Felicia

AU - Brown, Michael G.

AU - Fu, Shu Man

PY - 2006/11/15

Y1 - 2006/11/15

N2 - The genetic and environmental factors that control the development of Sjögren's syndrome, an autoimmune disease mainly involving the salivary and lacrimal glands, are poorly understood. Viruses which infect the glands may act as a trigger for disease. The ability of sialotropic murine CMV (MCMV) to induce acute and chronic glandular disease was characterized in an autoimmune-prone mouse strain, NZM2328. MCMV levels were detectable in the salivary and lacrimal glands 14-28 days after i.p. infection and correlated with acute inflammation in the submandibular gland. After latency, virus was undetectable in the glands by PCR. At this stage, NZM2328 female mice developed severe chronic periductal inflammation in both submandibular and lacrimal glands in contrast to the much milder infiltrates found in female B6-lpr and male NZM2328. The focal infiltrates consisted of CD4+ and B220+ cells as opposed to diffuse CD4+, CD8+, and B220 + cells during acute infection. Salivary gland functional studies revealed a gender-specific progressive loss of secretory function between days 90 and 125 postinfection. Latent MCMV infection did not significantly affect the low incidence of autoantibodies to Ro/SSA and La/SSB Ags in NZM2328 mice. However, reactivities to other salivary and lacrimal gland proteins were readily detected. MCMV infection did not significantly alter the spontaneous onset of kidney disease in NZM2328. Thus, chronic inflammation induced by MCMV with decreased secretory function in NZM2328 mice resembles the disease manifestations of human Sjögren's syndrome.

AB - The genetic and environmental factors that control the development of Sjögren's syndrome, an autoimmune disease mainly involving the salivary and lacrimal glands, are poorly understood. Viruses which infect the glands may act as a trigger for disease. The ability of sialotropic murine CMV (MCMV) to induce acute and chronic glandular disease was characterized in an autoimmune-prone mouse strain, NZM2328. MCMV levels were detectable in the salivary and lacrimal glands 14-28 days after i.p. infection and correlated with acute inflammation in the submandibular gland. After latency, virus was undetectable in the glands by PCR. At this stage, NZM2328 female mice developed severe chronic periductal inflammation in both submandibular and lacrimal glands in contrast to the much milder infiltrates found in female B6-lpr and male NZM2328. The focal infiltrates consisted of CD4+ and B220+ cells as opposed to diffuse CD4+, CD8+, and B220 + cells during acute infection. Salivary gland functional studies revealed a gender-specific progressive loss of secretory function between days 90 and 125 postinfection. Latent MCMV infection did not significantly affect the low incidence of autoantibodies to Ro/SSA and La/SSB Ags in NZM2328 mice. However, reactivities to other salivary and lacrimal gland proteins were readily detected. MCMV infection did not significantly alter the spontaneous onset of kidney disease in NZM2328. Thus, chronic inflammation induced by MCMV with decreased secretory function in NZM2328 mice resembles the disease manifestations of human Sjögren's syndrome.

UR - http://www.scopus.com/inward/record.url?scp=33750807886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750807886&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.177.10.7391

DO - 10.4049/jimmunol.177.10.7391

M3 - Article

VL - 177

SP - 7391

EP - 7397

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 10

ER -