Sexually dimorphic expression of Dax-1 in the adrenal cortex

Tokuo Mukai, Masatomo Kusaka, Ken Kawabe, Kiminobu Goto, Hajime Nawata, Kenji Fujieda, Ken-Ichirou Morohashi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: The DAX-1 (also known as AHC) gene encodes an unusual member of the nuclear receptor superfamily, and the gene product plays a pivotal role during gonadal and adrenal differentiation. Mutations of the human DAX-1 gene cause X-linked adrenal hypoplasia congenita associated with hypogonadotropic hypogonadism. However, the molecular mechanisms underlying the transcriptional regulation of the gene are not well understood. Results: Sexually dimorphic expression of Dax-1 (NR0B1) in the adrenal cortex was observed by RT-PCR, Western blotting and immunohistochemistry. The differential expression was abolished by gonadectomy and was restored again by sex steroid replacement. Our results suggested that the Dax-1 gene transcription is suppressed by androgens and and androgen receptor (AR/NR3C4). Dax-1 gene transcription is regulated by Ad4BP/SF-1 (NR5A1), therefore we investigated the functional correlation between A4BP/SF-1 and AR. Interestingly, AR down-regulated the Dax-1 gene transcription mediated by Ad4BP/SF-1 in the presence of the ligand. DNA binding by AR was not essential for the suppressive action, however the suppression seemed to be dependent on the promoter contexts. An interaction between Ad4BP/SF-1 and AR was detected in the presence of the ligand for AR. Conclusion: The present study revealed that the expression of Dax-1 in the adrenal cortex is regulated by androgen and the receptor. Interestingly, AR acts as a suppressor in the presence of the ligand through interaction with Ad4BP/SF-1.

Original languageEnglish
Pages (from-to)717-729
Number of pages13
JournalGenes to Cells
Volume7
Issue number7
DOIs
Publication statusPublished - Aug 7 2002

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Adrenal Cortex
Genes
Androgen Receptors
Ligands
Hypogonadism
Cytoplasmic and Nuclear Receptors
Androgens
Western Blotting
Immunohistochemistry
Steroids
Polymerase Chain Reaction
Mutation
DNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Mukai, T., Kusaka, M., Kawabe, K., Goto, K., Nawata, H., Fujieda, K., & Morohashi, K-I. (2002). Sexually dimorphic expression of Dax-1 in the adrenal cortex. Genes to Cells, 7(7), 717-729. https://doi.org/10.1046/j.1365-2443.2002.00556.x

Sexually dimorphic expression of Dax-1 in the adrenal cortex. / Mukai, Tokuo; Kusaka, Masatomo; Kawabe, Ken; Goto, Kiminobu; Nawata, Hajime; Fujieda, Kenji; Morohashi, Ken-Ichirou.

In: Genes to Cells, Vol. 7, No. 7, 07.08.2002, p. 717-729.

Research output: Contribution to journalArticle

Mukai, T, Kusaka, M, Kawabe, K, Goto, K, Nawata, H, Fujieda, K & Morohashi, K-I 2002, 'Sexually dimorphic expression of Dax-1 in the adrenal cortex', Genes to Cells, vol. 7, no. 7, pp. 717-729. https://doi.org/10.1046/j.1365-2443.2002.00556.x
Mukai T, Kusaka M, Kawabe K, Goto K, Nawata H, Fujieda K et al. Sexually dimorphic expression of Dax-1 in the adrenal cortex. Genes to Cells. 2002 Aug 7;7(7):717-729. https://doi.org/10.1046/j.1365-2443.2002.00556.x
Mukai, Tokuo ; Kusaka, Masatomo ; Kawabe, Ken ; Goto, Kiminobu ; Nawata, Hajime ; Fujieda, Kenji ; Morohashi, Ken-Ichirou. / Sexually dimorphic expression of Dax-1 in the adrenal cortex. In: Genes to Cells. 2002 ; Vol. 7, No. 7. pp. 717-729.
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