Shift of low to high nucleic acid bacteria as a potential bioindicator for the screening of anthropogenic effects in a receiving river due to palm oil mill effluent final discharge

Siti Suhailah Sharuddin, Norhayati Ramli, Diana Mohd-Nor, Mohd Ali Hassan, Toshinari Maeda, Yoshihito Shirai, Kenji Sakai, Yukihiro Tashiro

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The microbiological effects of palm oil mill effluent (POME) final discharge upon a receiving river were assessed in this study by using the nucleic acid double staining assay based on flow cytometry. The functional status of the bacterial community at the single-cell level was determined with regards to their abundance, viability and nucleic acid content to monitor the effects of POME final discharge on the affected river. The effluent resulted in the increment of the total cell concentration (TCC) and viable cells which were correlated with the increment of biological oxygen demand (BOD 5 ) and total organic carbon (TOC) concentrations in the receiving river. The shift of low nucleic acid (LNA) to high nucleic acid (HNA) bacterial cells in the affected river suggested the transformation of dormant to active cells due to the POME final discharge. This is the first study to report on the shift of LNA/HNA ratios which may serves as a potential bioindicator in the screening of the anthropogenic effects due to POME final discharge in river water with originally high LNA proportions. Monitoring the effluent discharge at low trophic level using flow cytometry is a rapid and sensitive approach when compared to the current physicochemical assessment method. This approach allows for the screening of river water contamination caused by POME final discharge prior to a full assessment using the recently proposed specific bacterial indicators.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalEcological Indicators
Volume85
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

oil mill effluents
nucleic acid
bioindicator
anthropogenic effect
nucleic acids
anthropogenic activities
mill
effluent
screening
bacterium
rivers
oil
bacteria
river
biochemical oxygen demand
flow cytometry
river water
cells
effluents
functional status

All Science Journal Classification (ASJC) codes

  • Decision Sciences(all)
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Shift of low to high nucleic acid bacteria as a potential bioindicator for the screening of anthropogenic effects in a receiving river due to palm oil mill effluent final discharge. / Sharuddin, Siti Suhailah; Ramli, Norhayati; Mohd-Nor, Diana; Hassan, Mohd Ali; Maeda, Toshinari; Shirai, Yoshihito; Sakai, Kenji; Tashiro, Yukihiro.

In: Ecological Indicators, Vol. 85, 01.02.2018, p. 79-84.

Research output: Contribution to journalArticle

@article{e2edb361cc78433ea5ef43ee8e79b6c0,
title = "Shift of low to high nucleic acid bacteria as a potential bioindicator for the screening of anthropogenic effects in a receiving river due to palm oil mill effluent final discharge",
abstract = "The microbiological effects of palm oil mill effluent (POME) final discharge upon a receiving river were assessed in this study by using the nucleic acid double staining assay based on flow cytometry. The functional status of the bacterial community at the single-cell level was determined with regards to their abundance, viability and nucleic acid content to monitor the effects of POME final discharge on the affected river. The effluent resulted in the increment of the total cell concentration (TCC) and viable cells which were correlated with the increment of biological oxygen demand (BOD 5 ) and total organic carbon (TOC) concentrations in the receiving river. The shift of low nucleic acid (LNA) to high nucleic acid (HNA) bacterial cells in the affected river suggested the transformation of dormant to active cells due to the POME final discharge. This is the first study to report on the shift of LNA/HNA ratios which may serves as a potential bioindicator in the screening of the anthropogenic effects due to POME final discharge in river water with originally high LNA proportions. Monitoring the effluent discharge at low trophic level using flow cytometry is a rapid and sensitive approach when compared to the current physicochemical assessment method. This approach allows for the screening of river water contamination caused by POME final discharge prior to a full assessment using the recently proposed specific bacterial indicators.",
author = "Sharuddin, {Siti Suhailah} and Norhayati Ramli and Diana Mohd-Nor and Hassan, {Mohd Ali} and Toshinari Maeda and Yoshihito Shirai and Kenji Sakai and Yukihiro Tashiro",
year = "2018",
month = "2",
day = "1",
doi = "10.1016/j.ecolind.2017.10.020",
language = "English",
volume = "85",
pages = "79--84",
journal = "Ecological Indicators",
issn = "1470-160X",
publisher = "Elsevier",

}

TY - JOUR

T1 - Shift of low to high nucleic acid bacteria as a potential bioindicator for the screening of anthropogenic effects in a receiving river due to palm oil mill effluent final discharge

AU - Sharuddin, Siti Suhailah

AU - Ramli, Norhayati

AU - Mohd-Nor, Diana

AU - Hassan, Mohd Ali

AU - Maeda, Toshinari

AU - Shirai, Yoshihito

AU - Sakai, Kenji

AU - Tashiro, Yukihiro

PY - 2018/2/1

Y1 - 2018/2/1

N2 - The microbiological effects of palm oil mill effluent (POME) final discharge upon a receiving river were assessed in this study by using the nucleic acid double staining assay based on flow cytometry. The functional status of the bacterial community at the single-cell level was determined with regards to their abundance, viability and nucleic acid content to monitor the effects of POME final discharge on the affected river. The effluent resulted in the increment of the total cell concentration (TCC) and viable cells which were correlated with the increment of biological oxygen demand (BOD 5 ) and total organic carbon (TOC) concentrations in the receiving river. The shift of low nucleic acid (LNA) to high nucleic acid (HNA) bacterial cells in the affected river suggested the transformation of dormant to active cells due to the POME final discharge. This is the first study to report on the shift of LNA/HNA ratios which may serves as a potential bioindicator in the screening of the anthropogenic effects due to POME final discharge in river water with originally high LNA proportions. Monitoring the effluent discharge at low trophic level using flow cytometry is a rapid and sensitive approach when compared to the current physicochemical assessment method. This approach allows for the screening of river water contamination caused by POME final discharge prior to a full assessment using the recently proposed specific bacterial indicators.

AB - The microbiological effects of palm oil mill effluent (POME) final discharge upon a receiving river were assessed in this study by using the nucleic acid double staining assay based on flow cytometry. The functional status of the bacterial community at the single-cell level was determined with regards to their abundance, viability and nucleic acid content to monitor the effects of POME final discharge on the affected river. The effluent resulted in the increment of the total cell concentration (TCC) and viable cells which were correlated with the increment of biological oxygen demand (BOD 5 ) and total organic carbon (TOC) concentrations in the receiving river. The shift of low nucleic acid (LNA) to high nucleic acid (HNA) bacterial cells in the affected river suggested the transformation of dormant to active cells due to the POME final discharge. This is the first study to report on the shift of LNA/HNA ratios which may serves as a potential bioindicator in the screening of the anthropogenic effects due to POME final discharge in river water with originally high LNA proportions. Monitoring the effluent discharge at low trophic level using flow cytometry is a rapid and sensitive approach when compared to the current physicochemical assessment method. This approach allows for the screening of river water contamination caused by POME final discharge prior to a full assessment using the recently proposed specific bacterial indicators.

UR - http://www.scopus.com/inward/record.url?scp=85031735508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85031735508&partnerID=8YFLogxK

U2 - 10.1016/j.ecolind.2017.10.020

DO - 10.1016/j.ecolind.2017.10.020

M3 - Article

VL - 85

SP - 79

EP - 84

JO - Ecological Indicators

JF - Ecological Indicators

SN - 1470-160X

ER -