Siamycin attenuates fsr quorum sensing mediated by a gelatinase biosynthesis-activating pheromone in Enterococcus faecalis

Jiro Nakayama, Emi Tanaka, Reiko Kariyama, Koji Nagata, Kenzo Nishiguchi, Ritsuko Mitsuhata, Yumi Uemura, Masaru Tanokura, Hiromi Kumon, Kenji Sonomoto

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The expression of two Enterococcus faecalis virulence-related proteases, gelatinase (GelE) and serine protease (SprE), is positively regulated by a quorum-sensing system encoded by the fsr gene cluster. In this system, E. faecalis secretes an autoinducing peptide, gelatinase biosynthesis-activating pheromone (GBAP), which triggers the FsrC-FsrA two-component regulatory system controlling the expression of two transcripts, fsrBDC and gelE-sprE. In the present study, we screened actinomycete metabolites for inhibitors of fsr quorum sensing. E. faecalis was cultured with each actinomycete culture supernatant tested, and the production of gelatinase and the production of GBAP were examined using the first screening and the second screening, respectively. Culture supernatant of Streptomyces sp. strain Y33-1 had the most potent inhibitory effect on both gelatinase production and GBAP production without inhibiting E. faecalis cell growth. The inhibitor in the culture supernatant was identified as a known peptide antibiotic, siamycin I. Siamycin I inhibited both gelatinase production and GBAP production at submicromolar concentrations, and it inhibited E. faecalis cell growth at concentrations above micromolar concentrations. Quantitative analysis of fsrBDC and gelE-sprE transcripts revealed that siamycin I suppressed the expression of both transcripts at a sublethal concentration. Siamycin I attenuated gelatinase production even when an overdose of GBAP was exogenoasly added to the culture. These results suggested that siamycin I inhibited the GBAP signaling via the FsrC-FsrA two-component regulatory system in a noncompetitive manner. The sublethal concentrations of siamycin I also attenuated biofilm formation. Treatment with siamycin could be a novel means of treating enterococcal infections.

Original languageEnglish
Pages (from-to)1358-1365
Number of pages8
JournalJournal of bacteriology
Volume189
Issue number4
DOIs
Publication statusPublished - Feb 1 2007

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Quorum Sensing
Enterococcus faecalis
Gelatinases
Actinobacteria
Peptide Biosynthesis
Streptomyces
Serine Proteases
Biofilms
Multigene Family
Growth
Virulence
gelatinase biosynthesis-activating pheromone
siamycin I
Peptide Hydrolases
Anti-Bacterial Agents
Peptides
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Siamycin attenuates fsr quorum sensing mediated by a gelatinase biosynthesis-activating pheromone in Enterococcus faecalis. / Nakayama, Jiro; Tanaka, Emi; Kariyama, Reiko; Nagata, Koji; Nishiguchi, Kenzo; Mitsuhata, Ritsuko; Uemura, Yumi; Tanokura, Masaru; Kumon, Hiromi; Sonomoto, Kenji.

In: Journal of bacteriology, Vol. 189, No. 4, 01.02.2007, p. 1358-1365.

Research output: Contribution to journalArticle

Nakayama, J, Tanaka, E, Kariyama, R, Nagata, K, Nishiguchi, K, Mitsuhata, R, Uemura, Y, Tanokura, M, Kumon, H & Sonomoto, K 2007, 'Siamycin attenuates fsr quorum sensing mediated by a gelatinase biosynthesis-activating pheromone in Enterococcus faecalis', Journal of bacteriology, vol. 189, no. 4, pp. 1358-1365. https://doi.org/10.1128/JB.00969-06
Nakayama, Jiro ; Tanaka, Emi ; Kariyama, Reiko ; Nagata, Koji ; Nishiguchi, Kenzo ; Mitsuhata, Ritsuko ; Uemura, Yumi ; Tanokura, Masaru ; Kumon, Hiromi ; Sonomoto, Kenji. / Siamycin attenuates fsr quorum sensing mediated by a gelatinase biosynthesis-activating pheromone in Enterococcus faecalis. In: Journal of bacteriology. 2007 ; Vol. 189, No. 4. pp. 1358-1365.
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