SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells

Isao Kobayashi, Haruna Tsukioka, Natuo Kômoto, Keiro Uchino, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe, Shuichiro Tomita

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

RNA interference is one of the most revolutionary tools in the study of gene function, particularly in non-model systems. However, in Bombyx mori, as with many lepidopteran species, attempts at systemic RNAi have had mixed success. Gene identification and phylogenetic analyses suggest that Bombyx has the core RNAi machinery, which is necessary to undergo RNAi as a cellular response. We introduced sid genes from Caenorhabditis elegans into Bombyx BmN4 cells to enhance the uptake of dsRNA and revealed that the SID-1 protein, but not SID-2, has the ability to endow the RNAi effect with the addition of dsRNA to the medium. Observed RNAi effect was dependent on both the levels of sid-1 expression and the concentration of the dsRNA. These results suggest that SID-1 promotes the uptake of dsRNA from the medium into Bombyx cells. We generated transgenic animals that express sid-1 but have not detected significant enhancements of invivo phenotype in response to the injection of the dsRNA into hemocoel.

Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume42
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

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