SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells

Isao Kobayashi, Haruna Tsukioka, Natuo Kômoto, Keiro Uchino, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe, Shuichiro Tomita

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

RNA interference is one of the most revolutionary tools in the study of gene function, particularly in non-model systems. However, in Bombyx mori, as with many lepidopteran species, attempts at systemic RNAi have had mixed success. Gene identification and phylogenetic analyses suggest that Bombyx has the core RNAi machinery, which is necessary to undergo RNAi as a cellular response. We introduced sid genes from Caenorhabditis elegans into Bombyx BmN4 cells to enhance the uptake of dsRNA and revealed that the SID-1 protein, but not SID-2, has the ability to endow the RNAi effect with the addition of dsRNA to the medium. Observed RNAi effect was dependent on both the levels of sid-1 expression and the concentration of the dsRNA. These results suggest that SID-1 promotes the uptake of dsRNA from the medium into Bombyx cells. We generated transgenic animals that express sid-1 but have not detected significant enhancements of invivo phenotype in response to the injection of the dsRNA into hemocoel.

Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume42
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

Fingerprint

Caenorhabditis elegans Proteins
Bombyx
Sudden Infant Death
double-stranded RNA
Caenorhabditis elegans
RNA Interference
Genes
uptake mechanisms
proteins
cells
Machinery
Animals
hemocoel
transgenic animals
RNA
genes
Bombyx mori
Genetically Modified Animals
RNA interference
Lepidoptera

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Kobayashi, I., Tsukioka, H., Kômoto, N., Uchino, K., Sezutsu, H., Tamura, T., ... Tomita, S. (2012). SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells. Insect Biochemistry and Molecular Biology, 42(2), 148-154. https://doi.org/10.1016/j.ibmb.2011.11.007

SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells. / Kobayashi, Isao; Tsukioka, Haruna; Kômoto, Natuo; Uchino, Keiro; Sezutsu, Hideki; Tamura, Toshiki; Kusakabe, Takahiro; Tomita, Shuichiro.

In: Insect Biochemistry and Molecular Biology, Vol. 42, No. 2, 01.02.2012, p. 148-154.

Research output: Contribution to journalArticle

Kobayashi, I, Tsukioka, H, Kômoto, N, Uchino, K, Sezutsu, H, Tamura, T, Kusakabe, T & Tomita, S 2012, 'SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells', Insect Biochemistry and Molecular Biology, vol. 42, no. 2, pp. 148-154. https://doi.org/10.1016/j.ibmb.2011.11.007
Kobayashi, Isao ; Tsukioka, Haruna ; Kômoto, Natuo ; Uchino, Keiro ; Sezutsu, Hideki ; Tamura, Toshiki ; Kusakabe, Takahiro ; Tomita, Shuichiro. / SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells. In: Insect Biochemistry and Molecular Biology. 2012 ; Vol. 42, No. 2. pp. 148-154.
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