Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials

Takeshi Mori, Go Yamanouchi, Xiaoming Han, Yusuke Inoue, Syuhei Shigaki, Takayuki Yamaji, Tatsuhiko Sonoda, Kei Yasui, Hisato Hayashi, Takuro Niidome, Yoshiki Katayama

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Abstract

The fabrication of peptide microarrays using hyperbranched polymers (HBPs) to improve the signal-to-noise ratio was demonstrated. Due to a high density of reactive groups at the chain ends of the HBPs, as well as to their spherical shape, HBPs can be used as linkers to increase the amount of immobilized peptides through raising the specific surface area of the glass substrate. A zwitterionic HBP was used as a blocking agent to reduce the noise level of the peptide microarrays. The zwitterionic HBP shows comparably excellent blocking ability to a commercially available BSA-based blocking agent. Thus, it was concluded that HBPs have high potential for the fabrication of highly sensitive peptide microarrays.

Original languageEnglish
Article number102020
JournalJournal of Applied Physics
Volume105
Issue number10
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

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    Mori, T., Yamanouchi, G., Han, X., Inoue, Y., Shigaki, S., Yamaji, T., Sonoda, T., Yasui, K., Hayashi, H., Niidome, T., & Katayama, Y. (2009). Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials. Journal of Applied Physics, 105(10), [102020]. https://doi.org/10.1063/1.3116124