Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials

Takeshi Mori; Go Yamanouchi; Xiaoming Han; Yusuke Inoue; Syuhei Shigaki; Takayuki Yamaji; Tatsuhiko Sonoda; Kei Yasui; Hisato Hayashi; Takuro Niidome; Yoshiki Katayama

doi:10.1063/1.3116124

Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials

Takeshi Mori, Go Yamanouchi, Xiaoming Han, Yusuke Inoue, Syuhei Shigaki, Takayuki Yamaji, Tatsuhiko Sonoda, Kei Yasui, Hisato Hayashi, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The fabrication of peptide microarrays using hyperbranched polymers (HBPs) to improve the signal-to-noise ratio was demonstrated. Due to a high density of reactive groups at the chain ends of the HBPs, as well as to their spherical shape, HBPs can be used as linkers to increase the amount of immobilized peptides through raising the specific surface area of the glass substrate. A zwitterionic HBP was used as a blocking agent to reduce the noise level of the peptide microarrays. The zwitterionic HBP shows comparably excellent blocking ability to a commercially available BSA-based blocking agent. Thus, it was concluded that HBPs have high potential for the fabrication of highly sensitive peptide microarrays.

Original language	English
Article number	102020
Journal	Journal of Applied Physics
Volume	105
Issue number	10
DOIs	https://doi.org/10.1063/1.3116124
Publication status	Published - 2009

All Science Journal Classification (ASJC) codes

Physics and Astronomy(all)

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10.1063/1.3116124

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title = "Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials",

abstract = "The fabrication of peptide microarrays using hyperbranched polymers (HBPs) to improve the signal-to-noise ratio was demonstrated. Due to a high density of reactive groups at the chain ends of the HBPs, as well as to their spherical shape, HBPs can be used as linkers to increase the amount of immobilized peptides through raising the specific surface area of the glass substrate. A zwitterionic HBP was used as a blocking agent to reduce the noise level of the peptide microarrays. The zwitterionic HBP shows comparably excellent blocking ability to a commercially available BSA-based blocking agent. Thus, it was concluded that HBPs have high potential for the fabrication of highly sensitive peptide microarrays.",

author = "Takeshi Mori and Go Yamanouchi and Xiaoming Han and Yusuke Inoue and Syuhei Shigaki and Takayuki Yamaji and Tatsuhiko Sonoda and Kei Yasui and Hisato Hayashi and Takuro Niidome and Yoshiki Katayama",

note = "Funding Information: This work was supported by the New Energy and Industrial Technology (NEDO), CREST from the Japan Science and Technology Agency, and a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science, and Technology.",

year = "2009",

doi = "10.1063/1.3116124",

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T1 - Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials

AU - Mori, Takeshi

AU - Yamanouchi, Go

AU - Han, Xiaoming

AU - Inoue, Yusuke

AU - Shigaki, Syuhei

AU - Yamaji, Takayuki

AU - Sonoda, Tatsuhiko

AU - Yasui, Kei

AU - Hayashi, Hisato

AU - Niidome, Takuro

AU - Katayama, Yoshiki

N1 - Funding Information: This work was supported by the New Energy and Industrial Technology (NEDO), CREST from the Japan Science and Technology Agency, and a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science, and Technology.

PY - 2009

Y1 - 2009

N2 - The fabrication of peptide microarrays using hyperbranched polymers (HBPs) to improve the signal-to-noise ratio was demonstrated. Due to a high density of reactive groups at the chain ends of the HBPs, as well as to their spherical shape, HBPs can be used as linkers to increase the amount of immobilized peptides through raising the specific surface area of the glass substrate. A zwitterionic HBP was used as a blocking agent to reduce the noise level of the peptide microarrays. The zwitterionic HBP shows comparably excellent blocking ability to a commercially available BSA-based blocking agent. Thus, it was concluded that HBPs have high potential for the fabrication of highly sensitive peptide microarrays.

AB - The fabrication of peptide microarrays using hyperbranched polymers (HBPs) to improve the signal-to-noise ratio was demonstrated. Due to a high density of reactive groups at the chain ends of the HBPs, as well as to their spherical shape, HBPs can be used as linkers to increase the amount of immobilized peptides through raising the specific surface area of the glass substrate. A zwitterionic HBP was used as a blocking agent to reduce the noise level of the peptide microarrays. The zwitterionic HBP shows comparably excellent blocking ability to a commercially available BSA-based blocking agent. Thus, it was concluded that HBPs have high potential for the fabrication of highly sensitive peptide microarrays.

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JF - Journal of Applied Physics

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Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials

Abstract

All Science Journal Classification (ASJC) codes

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