Significance of donor nutritional status for rewarming injury of the hepatic graft in rats

S. Wakiyama, Katsuhiko Yanaga, Yuji Soejima, K. Sugimachi, A. Scheule

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We studied the significance of donor nutritional status for hepatic rewarming injury and the usefulness of the glucagon loading test for the assessment of donor nutritional status in rats. In experiment 1 the animals were either free fed or fasted for 6, 24 or 48 h. The livers were preserved in chilled lactated Ringer’s solution for 4 h and were divided into eight groups according to the fasting and rewarming periods. The ammonia level in the graft effluent was increased in grafts preserved for 24 h or longer, which was augmented when rewarming time was increased from 15 to 30 min (p n 0.05). In experiment 2, the animals were divided into four groups (n = 3 each) according to the fasting period. The hepatic tissue glycogen content was measured after fasting, and the serum glucose was measured after the administration of 50 µg/kg of glucagon i.v. The hepatic tissue glycogen content correlated with the increase rate of serum glucose (p n 0.0001). We conclude that glycogen may protect the hepatic graft from rewarming injury by improving energy status, particularly in prolonged rewarming. The glucagon loading test seems to be useful for assessing the glycogen content of the hepatic graft.

Original languageEnglish
Pages (from-to)339-345
Number of pages7
JournalEuropean Surgical Research
Volume29
Issue number5
DOIs
Publication statusPublished - Jan 1 1997

Fingerprint

Rewarming
Nutritional Status
Liver Glycogen
Transplants
Glucagon
Liver
Wounds and Injuries
Fasting
Glucose
Serum
Glycogen
Ammonia

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Significance of donor nutritional status for rewarming injury of the hepatic graft in rats. / Wakiyama, S.; Yanaga, Katsuhiko; Soejima, Yuji; Sugimachi, K.; Scheule, A.

In: European Surgical Research, Vol. 29, No. 5, 01.01.1997, p. 339-345.

Research output: Contribution to journalArticle

Wakiyama, S. ; Yanaga, Katsuhiko ; Soejima, Yuji ; Sugimachi, K. ; Scheule, A. / Significance of donor nutritional status for rewarming injury of the hepatic graft in rats. In: European Surgical Research. 1997 ; Vol. 29, No. 5. pp. 339-345.
@article{d6383f3ffbdc45379014566501016fd3,
title = "Significance of donor nutritional status for rewarming injury of the hepatic graft in rats",
abstract = "We studied the significance of donor nutritional status for hepatic rewarming injury and the usefulness of the glucagon loading test for the assessment of donor nutritional status in rats. In experiment 1 the animals were either free fed or fasted for 6, 24 or 48 h. The livers were preserved in chilled lactated Ringer’s solution for 4 h and were divided into eight groups according to the fasting and rewarming periods. The ammonia level in the graft effluent was increased in grafts preserved for 24 h or longer, which was augmented when rewarming time was increased from 15 to 30 min (p n 0.05). In experiment 2, the animals were divided into four groups (n = 3 each) according to the fasting period. The hepatic tissue glycogen content was measured after fasting, and the serum glucose was measured after the administration of 50 µg/kg of glucagon i.v. The hepatic tissue glycogen content correlated with the increase rate of serum glucose (p n 0.0001). We conclude that glycogen may protect the hepatic graft from rewarming injury by improving energy status, particularly in prolonged rewarming. The glucagon loading test seems to be useful for assessing the glycogen content of the hepatic graft.",
author = "S. Wakiyama and Katsuhiko Yanaga and Yuji Soejima and K. Sugimachi and A. Scheule",
year = "1997",
month = "1",
day = "1",
doi = "10.1159/000129541",
language = "English",
volume = "29",
pages = "339--345",
journal = "European Surgical Research",
issn = "0014-312X",
publisher = "S. Karger AG",
number = "5",

}

TY - JOUR

T1 - Significance of donor nutritional status for rewarming injury of the hepatic graft in rats

AU - Wakiyama, S.

AU - Yanaga, Katsuhiko

AU - Soejima, Yuji

AU - Sugimachi, K.

AU - Scheule, A.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - We studied the significance of donor nutritional status for hepatic rewarming injury and the usefulness of the glucagon loading test for the assessment of donor nutritional status in rats. In experiment 1 the animals were either free fed or fasted for 6, 24 or 48 h. The livers were preserved in chilled lactated Ringer’s solution for 4 h and were divided into eight groups according to the fasting and rewarming periods. The ammonia level in the graft effluent was increased in grafts preserved for 24 h or longer, which was augmented when rewarming time was increased from 15 to 30 min (p n 0.05). In experiment 2, the animals were divided into four groups (n = 3 each) according to the fasting period. The hepatic tissue glycogen content was measured after fasting, and the serum glucose was measured after the administration of 50 µg/kg of glucagon i.v. The hepatic tissue glycogen content correlated with the increase rate of serum glucose (p n 0.0001). We conclude that glycogen may protect the hepatic graft from rewarming injury by improving energy status, particularly in prolonged rewarming. The glucagon loading test seems to be useful for assessing the glycogen content of the hepatic graft.

AB - We studied the significance of donor nutritional status for hepatic rewarming injury and the usefulness of the glucagon loading test for the assessment of donor nutritional status in rats. In experiment 1 the animals were either free fed or fasted for 6, 24 or 48 h. The livers were preserved in chilled lactated Ringer’s solution for 4 h and were divided into eight groups according to the fasting and rewarming periods. The ammonia level in the graft effluent was increased in grafts preserved for 24 h or longer, which was augmented when rewarming time was increased from 15 to 30 min (p n 0.05). In experiment 2, the animals were divided into four groups (n = 3 each) according to the fasting period. The hepatic tissue glycogen content was measured after fasting, and the serum glucose was measured after the administration of 50 µg/kg of glucagon i.v. The hepatic tissue glycogen content correlated with the increase rate of serum glucose (p n 0.0001). We conclude that glycogen may protect the hepatic graft from rewarming injury by improving energy status, particularly in prolonged rewarming. The glucagon loading test seems to be useful for assessing the glycogen content of the hepatic graft.

UR - http://www.scopus.com/inward/record.url?scp=0030883545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030883545&partnerID=8YFLogxK

U2 - 10.1159/000129541

DO - 10.1159/000129541

M3 - Article

VL - 29

SP - 339

EP - 345

JO - European Surgical Research

JF - European Surgical Research

SN - 0014-312X

IS - 5

ER -