Silencing Id-1 inhibits lymphangiogenesis through down-regulation of VEGF-C in oral squamous cell carcinoma

Zuoqing Dong, Fengcai Wei, Chengjun Zhou, Tomoki Sumida, Hiroyuki Hamakawa, Yingwei Hu, Shaohua Liu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Our previous study demonstrated that overexpression of Id-1 (inhibitor of differentiation/DNA binding) was associated with lymphatic metastasis in human oral squamous cell carcinoma (OSCC). In this study, we further unveiled the association of Id-1 with vascular endothelial growth factor-C (VEGF-C) and peritumoral lymphatic vessel density (PLVD), and the effect of silencing Id-1 on inhibiting lymphangiogenesis in OSCC. We found that Id-1 was associated with VEGF-C (r = 0.569, p < 0.001) and PLVD (r = 0.240, p < 0.001) in OSCC. Lentivirus-mediated RNA interference targeting Id-1 in an OSCC cell line Tca8113 resulted in down-regulation of VEGF-C (p = 0.003, 0.007). Moreover, when Id-1 was suppressed by injecting Id-1-siRNA-lentivirus into the transplanted tumors in nude mice, VEGF-C was down-regulated (p = 0.018) and the PLVD decreased (p = 0.001). Our results suggest that Id-1 was correlated with lymphangiogenesis in OSCC. Silencing Id-1 could inhibit lymphangiogenesis through down-regulation of VEGF-C and it might be a promising treatment modality for the lymphatic metastasis of OSCC.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalOral Oncology
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

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All Science Journal Classification (ASJC) codes

  • Oral Surgery
  • Oncology
  • Cancer Research

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