Silencing Id-1 with RNA interference inhibits adenoid cystic carcinoma in mice

Zhenggang Chen, Shaohua Liu, Tomoki Sumida, Shanzhen Sun, Yuan Wei, Meng Liu, Zuoqing Dong, Fan Zhang, Hiroyuki Hamakawa, Fengcai Wei

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The helix-loop-helix (HLH) protein Id-1 (inhibitor of DNA binding/differentiation) has been demonstrated to play an important role in tumor development. Our previous in vitro research has shown that Id-1 is a potential target in the treatment of human adenoid cystic carcinoma (ACCM). The purpose of this study was to analyze the influence of Id inhibition on ACCM in mice. Materials and Methods: To suppress the expression of Id-1 gene, we used lentivirus-mediated RNA interference to silence the Id-1 gene post-transcriptionally in ACCM models that stably express GFP in mice. Tumor development was evaluated by size measurement. Effects of Id-1 siRNA on mRNA and protein expression of Id-1 were analyzed using quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting respectively. Ki-67 expression was measured by immunohistochemistry. In vitro studies of Hoechst staining for cell apoptosis, Boyden-chamber assay for cell invasion, and MTT-tests for cell growth were performed as well. Results: Id-1 knockdown resulted in inhibition of tumor growth in mice. Id-1 siRNA significantly decreased not only Id-1 in mRNA and protein level, but also Ki-67 expression. In addition, apoptosis was induced and cell proliferation activity and invasion were significantly reduced. Conclusions: Lentivirus-mediated gene knockdown by silencing Id-1 constitute a valid methodological approach, which may represent an attractive, potent and specific therapeutic tool for the treatment of ACCM.

Original languageEnglish
Pages (from-to)57-66
Number of pages10
JournalJournal of Surgical Research
Volume169
Issue number1
DOIs
Publication statusPublished - Jul 1 2011
Externally publishedYes

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Adenoid Cystic Carcinoma
RNA Interference
Lentivirus
Inhibitor of Differentiation Protein 1
Small Interfering RNA
Apoptosis
Gene Knockdown Techniques
Neoplasms
Messenger RNA
Gene Silencing
Growth
Reverse Transcriptase Polymerase Chain Reaction
Genes
Proteins
Therapeutics
Western Blotting
Immunohistochemistry
Cell Proliferation
Staining and Labeling
Research

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Silencing Id-1 with RNA interference inhibits adenoid cystic carcinoma in mice. / Chen, Zhenggang; Liu, Shaohua; Sumida, Tomoki; Sun, Shanzhen; Wei, Yuan; Liu, Meng; Dong, Zuoqing; Zhang, Fan; Hamakawa, Hiroyuki; Wei, Fengcai.

In: Journal of Surgical Research, Vol. 169, No. 1, 01.07.2011, p. 57-66.

Research output: Contribution to journalArticle

Chen, Z, Liu, S, Sumida, T, Sun, S, Wei, Y, Liu, M, Dong, Z, Zhang, F, Hamakawa, H & Wei, F 2011, 'Silencing Id-1 with RNA interference inhibits adenoid cystic carcinoma in mice', Journal of Surgical Research, vol. 169, no. 1, pp. 57-66. https://doi.org/10.1016/j.jss.2009.11.723
Chen, Zhenggang ; Liu, Shaohua ; Sumida, Tomoki ; Sun, Shanzhen ; Wei, Yuan ; Liu, Meng ; Dong, Zuoqing ; Zhang, Fan ; Hamakawa, Hiroyuki ; Wei, Fengcai. / Silencing Id-1 with RNA interference inhibits adenoid cystic carcinoma in mice. In: Journal of Surgical Research. 2011 ; Vol. 169, No. 1. pp. 57-66.
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