Abstract
To develop effective methods for delivering steroid hormones to red seabream, we investigated the utility of dimethyl sulfoxide (DMSO) as a solvent for steroid hormones by examining the in vitro and in vivo release of estradiol-17β (E2) and 11-ketotestosterone (11-KT) from silicone capsules. In vitro E2 and 11-KT release rates from capsules containing steroid hormones in castor oil were proportional to the concentration of steroids within capsules. Increasing the proportion of DMSO to castor oil increased sex steroid hormone release from the capsules. The in vivo implantation of a single capsule containing 400 μg of E2 in 50% DMSO or 11-KT in 25% DMSO effectively increased serum levels of each steroid hormone in juvenile red seabream for a month. These results demonstrate that use of silicone capsules containing steroids in DMSO provides a useful method for delivering controlled and easily manipulated physiological doses of E2 and 11-KT to large fish such as red seabream.
| Original language | English |
|---|---|
| Pages (from-to) | 485-496 |
| Number of pages | 12 |
| Journal | Aquaculture |
| Volume | 239 |
| Issue number | 1-4 |
| DOIs | |
| Publication status | Published - Sep 30 2004 |
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All Science Journal Classification (ASJC) codes
- Aquatic Science
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Silicone implants for delivery of estradiol-17β and 11-ketotestosterone to red seabream Pagrus major. / Yamaguchi, Sonoko; Kagawa, Hirohiko; Gen, Koichiro; Okuzawa, Koichi; Matsuyama, Michiya.
In: Aquaculture, Vol. 239, No. 1-4, 30.09.2004, p. 485-496.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Silicone implants for delivery of estradiol-17β and 11-ketotestosterone to red seabream Pagrus major
AU - Yamaguchi, Sonoko
AU - Kagawa, Hirohiko
AU - Gen, Koichiro
AU - Okuzawa, Koichi
AU - Matsuyama, Michiya
PY - 2004/9/30
Y1 - 2004/9/30
N2 - To develop effective methods for delivering steroid hormones to red seabream, we investigated the utility of dimethyl sulfoxide (DMSO) as a solvent for steroid hormones by examining the in vitro and in vivo release of estradiol-17β (E2) and 11-ketotestosterone (11-KT) from silicone capsules. In vitro E2 and 11-KT release rates from capsules containing steroid hormones in castor oil were proportional to the concentration of steroids within capsules. Increasing the proportion of DMSO to castor oil increased sex steroid hormone release from the capsules. The in vivo implantation of a single capsule containing 400 μg of E2 in 50% DMSO or 11-KT in 25% DMSO effectively increased serum levels of each steroid hormone in juvenile red seabream for a month. These results demonstrate that use of silicone capsules containing steroids in DMSO provides a useful method for delivering controlled and easily manipulated physiological doses of E2 and 11-KT to large fish such as red seabream.
AB - To develop effective methods for delivering steroid hormones to red seabream, we investigated the utility of dimethyl sulfoxide (DMSO) as a solvent for steroid hormones by examining the in vitro and in vivo release of estradiol-17β (E2) and 11-ketotestosterone (11-KT) from silicone capsules. In vitro E2 and 11-KT release rates from capsules containing steroid hormones in castor oil were proportional to the concentration of steroids within capsules. Increasing the proportion of DMSO to castor oil increased sex steroid hormone release from the capsules. The in vivo implantation of a single capsule containing 400 μg of E2 in 50% DMSO or 11-KT in 25% DMSO effectively increased serum levels of each steroid hormone in juvenile red seabream for a month. These results demonstrate that use of silicone capsules containing steroids in DMSO provides a useful method for delivering controlled and easily manipulated physiological doses of E2 and 11-KT to large fish such as red seabream.
UR - http://www.scopus.com/inward/record.url?scp=7944234855&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7944234855&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2004.05.031
DO - 10.1016/j.aquaculture.2004.05.031
M3 - Article
AN - SCOPUS:7944234855
VL - 239
SP - 485
EP - 496
JO - Aquaculture
JF - Aquaculture
SN - 0044-8486
IS - 1-4
ER -