Silkworm midgut proteins interacting with a hemolymph protease inhibitor, CI-8

Yoshinori Ueno, Ningjia He, Minoru Ujita, Kohji Yamamoto, Yutaka Banno, Hiroshi Fujii, Yoichi Aso

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

CI-8 is the chymotrypsin inhibitor in hemolymph from the silkworm, Bombyx mori. It occurs in the midgut at the spinning stage of larva, but little information on the mechanism of its uptake in the midgut is available. We found that two polypeptides interacting with CI-8 are in the midgut membrane, and we purified them using a biotinylated CI-8, viz., p29 and p60, having molecular sizes of 29 kDa and 60 kDa respectively. The structures of p29 and p60 were examined by N-terminal amino acid sequencing and peptide mass mapping, including tryptic digestion. p29 was highly similar to the matured 19G1-30K lipoprotein from hemolymph, but p60 was novel. Purified p29 was recognized by anti-19G1-30K antibody, and was confirmed to be similar to 19G1-30K. The antibody also neutralized the CI-8 binding ability of p29 in the midgut membrane. p29 and p60 are perhaps proteinaceous factors involved in the uptake of CI-8 into the midgut through the membrane.

Original languageEnglish
Pages (from-to)1557-1563
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number7
DOIs
Publication statusPublished - Aug 1 2006

Fingerprint

Bombyx
Hemolymph
Protease Inhibitors
Membranes
Peptides
Proteins
Peptide Mapping
Antibodies
Protein Sequence Analysis
Chymotrypsin
Larva
Digestion
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Silkworm midgut proteins interacting with a hemolymph protease inhibitor, CI-8. / Ueno, Yoshinori; He, Ningjia; Ujita, Minoru; Yamamoto, Kohji; Banno, Yutaka; Fujii, Hiroshi; Aso, Yoichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 7, 01.08.2006, p. 1557-1563.

Research output: Contribution to journalArticle

Ueno, Yoshinori ; He, Ningjia ; Ujita, Minoru ; Yamamoto, Kohji ; Banno, Yutaka ; Fujii, Hiroshi ; Aso, Yoichi. / Silkworm midgut proteins interacting with a hemolymph protease inhibitor, CI-8. In: Bioscience, Biotechnology and Biochemistry. 2006 ; Vol. 70, No. 7. pp. 1557-1563.
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