Simple and effective label-free capillary electrophoretic analysis of sugars by complexation using quinoline boronic acids

Takuya Kubo, Koichi Kanemori, Risa Kusumoto, Takayuki Kawai, Kenji Sueyoshi, Toyohiro Naito, Koji Otsuka

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

An effective separation and detection procedure for sugars by capillary electrophoresis (CE) using a complexation between quinolineboronic acid (QBA) and multiple hydroxyl structure of sugar alcohol is reported. We investigated the variation of fluorescence spectra of a variety of QBAs with sorbitol at a wide range of pH conditions and then found that 5-isoQBA strongly enhanced the fluorescence intensity by the complexation at basic pH conditions. The other sugar alcohols having multiple hydroxyls also revealed the enhancement of the fluorescence intensity with 5-isoQBA, whereas the alternation of the intensity was not found in the sugars such as glucose. After optimization of the 5-isoQBA concentration and pH of the buffered solution in CE analysis, 6 sugar alcohols were successfully separated in the order based on the formation constants with 5-isoQBA, which were calculated from the variation of the fluorescence intensity with each sugar alcohol and 5-isoQBA. Furthermore, the limits of detection for sorbitol and xylitol by the CE method were estimated at 15 and 27 μM, respectively.

Original languageEnglish
Pages (from-to)5068-5073
Number of pages6
JournalAnalytical Chemistry
Volume87
Issue number10
DOIs
Publication statusPublished - May 19 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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