Simple and rapid determination of enflurane in human tissues using gas chromatography and gas chromatography-mass spectrometry

H. Ise, Keiko Kudo, N. Jitsufuchi, T. Imamura, Noriaki Ikeda

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A simple, rapid and reliable method was devised to determine the levels of enflurane in human tissues, using gas chromatography and gas chromatography-mass spectrometry. 1,4-Dioxane was used as an internal standard (I.S.). Enflurane and the I.S, were extracted from 0.25 g of body tissues using an automatic headspace sampler and 1 ml of headspace gas was injected into the gas chromatograph. Enflurane was analyzed qualitatively by gas chromatography-mass spectrometry and quantitatively by gas chromatography with a flame-ionization detector. The calibration curves in all tissues examined were linear in the concentration range 1-100 μg/0.25 g. The lower limit of detection was 200-300 ng/0.25 g. The accuracy and precision of this method were evaluated at two different concentrations, 1 and 20 μg/0.25 g. The coefficient of variation ranged from 3.4-13.4%. We used this method to determine the presence of enflurane in tissues from an autopsied individual who died suddenly during extirpation of a malignant tumor.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume698
Issue number1-2
DOIs
Publication statusPublished - Sep 26 1997

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Enflurane
Gas chromatography
Mass spectrometry
Tissue
Gases
Ionization
Tumors
Calibration
Detectors

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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Simple and rapid determination of enflurane in human tissues using gas chromatography and gas chromatography-mass spectrometry. / Ise, H.; Kudo, Keiko; Jitsufuchi, N.; Imamura, T.; Ikeda, Noriaki.

In: Journal of Chromatography B: Biomedical Applications, Vol. 698, No. 1-2, 26.09.1997, p. 97-102.

Research output: Contribution to journalArticle

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