Simple and sensitive determination of free and total morphine in human liver and kidney using gas chromatography-mass spectrometry

Keiko Kudo, Tomomi Ishida, Naoki Nishida, Naofumi Yoshioka, Hiromasa Inoue, Akiko Tsuji, Noriaki Ikeda

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We developed a reliable, simple and sensitive method to determine free and total morphine in human liver and kidney, using gas chromatography-mass spectrometry (GC-MS). Free morphine or total morphine obtained by acid hydrolysis from 0.2 g tissue sample was extracted using an Extrelut® NT column with an internal standard, dihydrocodeine, followed by trimethylsilylation. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of morphine in both liver and kidney samples were linear in the concentration range from 0.005 to 5 μg/g. The lower limits of detection of morphine were 0.005 μg/g. This method proved successful when we determined free and total morphine in liver and kidney obtained from an autopsied man who was mis-ingested morphine compound in the hospital, which resulted in the cause of death being morphine intoxication.

Original languageEnglish
Pages (from-to)359-363
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume830
Issue number2
DOIs
Publication statusPublished - Jan 18 2006

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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