Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using eLISA

Kiyoshi Noda, Kuniaki Tanaka, Akira Yamada, Jyunko Ogata, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A quantitative ELISA system was developed using a monoclonal antibody (MAb) specific for an antitumour immunoactive glycoprotein (CVS) derived from C. vulgaris strain CK22. The full measuring range of the assay extends from 0.63 to 10.0 ng/mL of CVS. Although no cross-reaction was observed to proteins tested or other biological response modifiers (BRMs) derived from different sources, cross-reactions were found with culture supernatants from two other strains of C. vulgaris having a strong antitumour immunoactivity. Treatment of CVS with protease, acid or alkali weakened or completely eliminated the reactivity against the MAb and also its antitumour immunoactivities. This ELISA system is suitable for the biologically active form of CVS derived from C. vulgaris strain CK22 and related immunoactive strains.

Original languageEnglish
Pages (from-to)581-585
Number of pages5
JournalPhytotherapy Research
Volume16
Issue number6
DOIs
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Pharmacology

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