Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis

Kozue Oana, Yoshiyuki Kawakami, Tetsuya Hayashi, Makoto Ohnishi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalMICROBIOLOGY and IMMUNOLOGY
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 1 2009
Externally publishedYes

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Pulsed Field Gel Electrophoresis
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis. / Oana, Kozue; Kawakami, Yoshiyuki; Hayashi, Tetsuya; Ohnishi, Makoto.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 53, No. 1, 01.01.2009, p. 45-48.

Research output: Contribution to journalArticle

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