Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis

Kozue Oana, Yoshiyuki Kawakami, Tetsuya Hayashi, Makoto Ohnishi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalMICROBIOLOGY and IMMUNOLOGY
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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