Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis

Kozue Oana; Yoshiyuki Kawakami; Tetsuya Hayashi; Makoto Ohnishi

doi:10.1111/j.1348-0421.2008.00084.x

Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis

Kozue Oana, Yoshiyuki Kawakami, Tetsuya Hayashi, Makoto Ohnishi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.

Original language	English
Pages (from-to)	45-48
Number of pages	4
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	53
Issue number	1
DOIs	https://doi.org/10.1111/j.1348-0421.2008.00084.x
Publication status	Published - Jan 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Virology

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abstract = "We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.",

author = "Kozue Oana and Yoshiyuki Kawakami and Tetsuya Hayashi and Makoto Ohnishi",

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AU - Kawakami, Yoshiyuki

AU - Hayashi, Tetsuya

AU - Ohnishi, Makoto

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