Abstract
We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.
| Original language | English |
|---|---|
| Pages (from-to) | 45-48 |
| Number of pages | 4 |
| Journal | MICROBIOLOGY and IMMUNOLOGY |
| Volume | 53 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2009 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Immunology
- Virology
Cite this
Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis. / Oana, Kozue; Kawakami, Yoshiyuki; Hayashi, Tetsuya; Ohnishi, Makoto.
In: MICROBIOLOGY and IMMUNOLOGY, Vol. 53, No. 1, 01.01.2009, p. 45-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simple broad-spectrum protocol using labiase for bacterial cell lysis to prepare genomic DNA for pulsed-field gel electrophoresis analysis
AU - Oana, Kozue
AU - Kawakami, Yoshiyuki
AU - Hayashi, Tetsuya
AU - Ohnishi, Makoto
PY - 2009/1/1
Y1 - 2009/1/1
N2 - We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.
AB - We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.
UR - http://www.scopus.com/inward/record.url?scp=61749083331&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61749083331&partnerID=8YFLogxK
U2 - 10.1111/j.1348-0421.2008.00084.x
DO - 10.1111/j.1348-0421.2008.00084.x
M3 - Article
C2 - 19161557
AN - SCOPUS:61749083331
VL - 53
SP - 45
EP - 48
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 1
ER -