Abstract
We have developed a simple broad-spectrum protocol using labiase for bacterial cell lysis in pulsed-field gel electrophoresis analysis. The protocol reported here is widely applicable to the preparations of genomic DNA from Gram-negative and -positive pathogens, including enterococcal strains resistant to any conventional lysis protocols.
Original language | English |
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Pages (from-to) | 45-48 |
Number of pages | 4 |
Journal | MICROBIOLOGY and IMMUNOLOGY |
Volume | 53 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2009 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Microbiology
- Immunology
- Virology