Simple surface treatment of cell-culture scaffolds with ultrafine bubble water

Hisao Matsuno, Toshiyuki Ohta, Atsuomi Shundo, Yasuo Fukunaga, Keiji Tanaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We propose a novel method to treat polymeric scaffold surfaces for cell culture with water containing nanobubbles, called ultrafine bubbles (UFBs), with typical diameters less than 1 μm. A thin film of polystyrene (PS) prepared on a solid substrate was exposed to UFB water for 2 days at room temperature. The PS surface was characterized by X-ray photoelectron spectroscopy (XPS), static contact angle measurements in water, and atomic force microscopy (AFM). The surface chemical composition and wettability of PS films remained unchanged after treatment, so that aggregation states of PS at film surfaces remained unaltered by UFB water. On the other hand, after treatment, many UFBs were adsorbed on hydrophobic PS surfaces. To study the effect of UFBs on scaffold properties, the adsorption behavior of fibronectin, which is a typical extracellular matrix protein involved in cell adhesion and proliferation, was examined. While the effect on the adsorption was unclear, the structural denaturation of fibronectin was enhanced after UFB treatment, so that the proliferation of fibroblast cells on PS surfaces was promoted.

Original languageEnglish
Pages (from-to)15238-15243
Number of pages6
JournalLangmuir
Volume30
Issue number50
DOIs
Publication statusPublished - Dec 23 2014

Fingerprint

Polystyrenes
Scaffolds (biology)
surface treatment
Cell culture
Surface treatment
polystyrene
bubbles
Water
water
Fibronectins
Adsorption
Denaturation
adsorption
biopolymer denaturation
Extracellular Matrix Proteins
Cell adhesion
fibroblasts
Cell proliferation
Fibroblasts
Angle measurement

All Science Journal Classification (ASJC) codes

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Simple surface treatment of cell-culture scaffolds with ultrafine bubble water. / Matsuno, Hisao; Ohta, Toshiyuki; Shundo, Atsuomi; Fukunaga, Yasuo; Tanaka, Keiji.

In: Langmuir, Vol. 30, No. 50, 23.12.2014, p. 15238-15243.

Research output: Contribution to journalArticle

@article{9cbb0b5a4e224aa192dea995ff1a179d,
title = "Simple surface treatment of cell-culture scaffolds with ultrafine bubble water",
abstract = "We propose a novel method to treat polymeric scaffold surfaces for cell culture with water containing nanobubbles, called ultrafine bubbles (UFBs), with typical diameters less than 1 μm. A thin film of polystyrene (PS) prepared on a solid substrate was exposed to UFB water for 2 days at room temperature. The PS surface was characterized by X-ray photoelectron spectroscopy (XPS), static contact angle measurements in water, and atomic force microscopy (AFM). The surface chemical composition and wettability of PS films remained unchanged after treatment, so that aggregation states of PS at film surfaces remained unaltered by UFB water. On the other hand, after treatment, many UFBs were adsorbed on hydrophobic PS surfaces. To study the effect of UFBs on scaffold properties, the adsorption behavior of fibronectin, which is a typical extracellular matrix protein involved in cell adhesion and proliferation, was examined. While the effect on the adsorption was unclear, the structural denaturation of fibronectin was enhanced after UFB treatment, so that the proliferation of fibroblast cells on PS surfaces was promoted.",
author = "Hisao Matsuno and Toshiyuki Ohta and Atsuomi Shundo and Yasuo Fukunaga and Keiji Tanaka",
year = "2014",
month = "12",
day = "23",
doi = "10.1021/la5035883",
language = "English",
volume = "30",
pages = "15238--15243",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "50",

}

TY - JOUR

T1 - Simple surface treatment of cell-culture scaffolds with ultrafine bubble water

AU - Matsuno, Hisao

AU - Ohta, Toshiyuki

AU - Shundo, Atsuomi

AU - Fukunaga, Yasuo

AU - Tanaka, Keiji

PY - 2014/12/23

Y1 - 2014/12/23

N2 - We propose a novel method to treat polymeric scaffold surfaces for cell culture with water containing nanobubbles, called ultrafine bubbles (UFBs), with typical diameters less than 1 μm. A thin film of polystyrene (PS) prepared on a solid substrate was exposed to UFB water for 2 days at room temperature. The PS surface was characterized by X-ray photoelectron spectroscopy (XPS), static contact angle measurements in water, and atomic force microscopy (AFM). The surface chemical composition and wettability of PS films remained unchanged after treatment, so that aggregation states of PS at film surfaces remained unaltered by UFB water. On the other hand, after treatment, many UFBs were adsorbed on hydrophobic PS surfaces. To study the effect of UFBs on scaffold properties, the adsorption behavior of fibronectin, which is a typical extracellular matrix protein involved in cell adhesion and proliferation, was examined. While the effect on the adsorption was unclear, the structural denaturation of fibronectin was enhanced after UFB treatment, so that the proliferation of fibroblast cells on PS surfaces was promoted.

AB - We propose a novel method to treat polymeric scaffold surfaces for cell culture with water containing nanobubbles, called ultrafine bubbles (UFBs), with typical diameters less than 1 μm. A thin film of polystyrene (PS) prepared on a solid substrate was exposed to UFB water for 2 days at room temperature. The PS surface was characterized by X-ray photoelectron spectroscopy (XPS), static contact angle measurements in water, and atomic force microscopy (AFM). The surface chemical composition and wettability of PS films remained unchanged after treatment, so that aggregation states of PS at film surfaces remained unaltered by UFB water. On the other hand, after treatment, many UFBs were adsorbed on hydrophobic PS surfaces. To study the effect of UFBs on scaffold properties, the adsorption behavior of fibronectin, which is a typical extracellular matrix protein involved in cell adhesion and proliferation, was examined. While the effect on the adsorption was unclear, the structural denaturation of fibronectin was enhanced after UFB treatment, so that the proliferation of fibroblast cells on PS surfaces was promoted.

UR - http://www.scopus.com/inward/record.url?scp=84919754664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84919754664&partnerID=8YFLogxK

U2 - 10.1021/la5035883

DO - 10.1021/la5035883

M3 - Article

AN - SCOPUS:84919754664

VL - 30

SP - 15238

EP - 15243

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 50

ER -