Simultaneous analysis of D-alanine, D-aspartic acid, and D-serine using chiral high-performance liquid chromatography-tandem mass spectrometry and its application to the rat plasma and tissues

Sachise Karakawa, Kazutaka Shimbo, Naoyuki Yamada, Toshimi Mizukoshi, Hiroshi Miyano, Masashi Mita, Wolfgang Lindner, Kenji Hamase

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A highly sensitive and selective chiral LC-MS/MS method for d-alanine, d-aspartic acid and d-serine has been developed using the precolumn derivatization reagents, 6-aminoquinolyl- N-hydroxysuccinimidyl carbamate (AccQ-Tag) or p-N,N,N-trimethylammonioanilyl N'-hydroxysuccinimidyl carbamate iodide (TAHS). The thus N-tagged enantiomers of the derivatized amino acids were nicely separated within 20. min using the cinchona alkaloid-based zwittterionic ion-exchange type enantioselective column, Chiralpak ZWIX(+). The selected reaction monitoring was applied for detecting the target d-amino acids in biological matrices. By using the present chiral LC-MS/MS method, the three d-amino acids and their l-forms could be simultaneously determined in the range of 0.1-500. nmol/mL. Finally, the technique was successfully applied to rat plasma and tissue samples.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume115
DOIs
Publication statusPublished - Nov 1 2015

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D-Aspartic Acid
High performance liquid chromatography
Tandem Mass Spectrometry
Alanine
Serine
Mass spectrometry
Rats
High Pressure Liquid Chromatography
Tissue
Plasmas
Amino Acids
Cinchona Alkaloids
Enantiomers
Ion Exchange
Aspartic Acid
Ion exchange
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Simultaneous analysis of D-alanine, D-aspartic acid, and D-serine using chiral high-performance liquid chromatography-tandem mass spectrometry and its application to the rat plasma and tissues. / Karakawa, Sachise; Shimbo, Kazutaka; Yamada, Naoyuki; Mizukoshi, Toshimi; Miyano, Hiroshi; Mita, Masashi; Lindner, Wolfgang; Hamase, Kenji.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 115, 01.11.2015, p. 123-129.

Research output: Contribution to journalArticle

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AU - Shimbo, Kazutaka

AU - Yamada, Naoyuki

AU - Mizukoshi, Toshimi

AU - Miyano, Hiroshi

AU - Mita, Masashi

AU - Lindner, Wolfgang

AU - Hamase, Kenji

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