Simultaneous analysis of D-alanine, D-aspartic acid, and D-serine using chiral high-performance liquid chromatography-tandem mass spectrometry and its application to the rat plasma and tissues

Sachise Karakawa, Kazutaka Shimbo, Naoyuki Yamada, Toshimi Mizukoshi, Hiroshi Miyano, Masashi Mita, Wolfgang Lindner, Kenji Hamase

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

A highly sensitive and selective chiral LC-MS/MS method for d-alanine, d-aspartic acid and d-serine has been developed using the precolumn derivatization reagents, 6-aminoquinolyl- N-hydroxysuccinimidyl carbamate (AccQ-Tag) or p-N,N,N-trimethylammonioanilyl N'-hydroxysuccinimidyl carbamate iodide (TAHS). The thus N-tagged enantiomers of the derivatized amino acids were nicely separated within 20. min using the cinchona alkaloid-based zwittterionic ion-exchange type enantioselective column, Chiralpak ZWIX(+). The selected reaction monitoring was applied for detecting the target d-amino acids in biological matrices. By using the present chiral LC-MS/MS method, the three d-amino acids and their l-forms could be simultaneously determined in the range of 0.1-500. nmol/mL. Finally, the technique was successfully applied to rat plasma and tissue samples.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume115
DOIs
Publication statusPublished - Nov 1 2015

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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