Abstract
Six peptides with various phosphorylation sensitivities for protein kinase A (PKA) were used for the simultaneous analysis of phosphorylated peptides using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. The mixture of six peptides was reacted with PKA and was analyzed by MALDI-TOF mass spectrometry. The intensity of all peaks except one phosphorylated peptide peak was very low (<20%). Moreover, we examined whether the addition of diammonium citrate to CHCA matrix at concentrations of 1-20 mg mL-1 can increase the peak intensity of peptides and phosphorylated peptides. The addition of diammonium citrate increased the peak intensity of peptides and phosphorylated peptides, but an increase in the intensity was unsatisfactory. Our study strongly suggests that MALDI-TOF mass spectrometry is not suitable for the simultaneous analysis of phosphorylated peptides.
| Original language | English |
|---|---|
| Pages (from-to) | 595-598 |
| Number of pages | 4 |
| Journal | Chromatographia |
| Volume | 63 |
| Issue number | 11-12 |
| DOIs | |
| Publication status | Published - Jun 1 2006 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Clinical Biochemistry
Cite this
Simultaneous analysis of phosphorylated peptides by MALDI-TOF-MS. / Kang, J. H.; Toita, R.; Jiang, Y.; Niidome, T.; Katayama, Yoshiki.
In: Chromatographia, Vol. 63, No. 11-12, 01.06.2006, p. 595-598.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous analysis of phosphorylated peptides by MALDI-TOF-MS
AU - Kang, J. H.
AU - Toita, R.
AU - Jiang, Y.
AU - Niidome, T.
AU - Katayama, Yoshiki
PY - 2006/6/1
Y1 - 2006/6/1
N2 - Six peptides with various phosphorylation sensitivities for protein kinase A (PKA) were used for the simultaneous analysis of phosphorylated peptides using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. The mixture of six peptides was reacted with PKA and was analyzed by MALDI-TOF mass spectrometry. The intensity of all peaks except one phosphorylated peptide peak was very low (<20%). Moreover, we examined whether the addition of diammonium citrate to CHCA matrix at concentrations of 1-20 mg mL-1 can increase the peak intensity of peptides and phosphorylated peptides. The addition of diammonium citrate increased the peak intensity of peptides and phosphorylated peptides, but an increase in the intensity was unsatisfactory. Our study strongly suggests that MALDI-TOF mass spectrometry is not suitable for the simultaneous analysis of phosphorylated peptides.
AB - Six peptides with various phosphorylation sensitivities for protein kinase A (PKA) were used for the simultaneous analysis of phosphorylated peptides using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. The mixture of six peptides was reacted with PKA and was analyzed by MALDI-TOF mass spectrometry. The intensity of all peaks except one phosphorylated peptide peak was very low (<20%). Moreover, we examined whether the addition of diammonium citrate to CHCA matrix at concentrations of 1-20 mg mL-1 can increase the peak intensity of peptides and phosphorylated peptides. The addition of diammonium citrate increased the peak intensity of peptides and phosphorylated peptides, but an increase in the intensity was unsatisfactory. Our study strongly suggests that MALDI-TOF mass spectrometry is not suitable for the simultaneous analysis of phosphorylated peptides.
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U2 - 10.1365/s10337-006-0810-1
DO - 10.1365/s10337-006-0810-1
M3 - Article
AN - SCOPUS:33745173102
VL - 63
SP - 595
EP - 598
JO - Chromatographia
JF - Chromatographia
SN - 0009-5893
IS - 11-12
ER -