Simultaneous analysis of phosphorylated peptides by MALDI-TOF-MS

J. H. Kang, R. Toita, Y. Jiang, T. Niidome, Yoshiki Katayama

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Six peptides with various phosphorylation sensitivities for protein kinase A (PKA) were used for the simultaneous analysis of phosphorylated peptides using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. The mixture of six peptides was reacted with PKA and was analyzed by MALDI-TOF mass spectrometry. The intensity of all peaks except one phosphorylated peptide peak was very low (<20%). Moreover, we examined whether the addition of diammonium citrate to CHCA matrix at concentrations of 1-20 mg mL-1 can increase the peak intensity of peptides and phosphorylated peptides. The addition of diammonium citrate increased the peak intensity of peptides and phosphorylated peptides, but an increase in the intensity was unsatisfactory. Our study strongly suggests that MALDI-TOF mass spectrometry is not suitable for the simultaneous analysis of phosphorylated peptides.

Original languageEnglish
Pages (from-to)595-598
Number of pages4
JournalChromatographia
Volume63
Issue number11-12
DOIs
Publication statusPublished - Jun 1 2006

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Ionization
Desorption
Lasers
Peptides
Mass spectrometry
Mass Spectrometry
Cyclic AMP-Dependent Protein Kinases
Citric Acid
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Simultaneous analysis of phosphorylated peptides by MALDI-TOF-MS. / Kang, J. H.; Toita, R.; Jiang, Y.; Niidome, T.; Katayama, Yoshiki.

In: Chromatographia, Vol. 63, No. 11-12, 01.06.2006, p. 595-598.

Research output: Contribution to journalArticle

Kang, J. H. ; Toita, R. ; Jiang, Y. ; Niidome, T. ; Katayama, Yoshiki. / Simultaneous analysis of phosphorylated peptides by MALDI-TOF-MS. In: Chromatographia. 2006 ; Vol. 63, No. 11-12. pp. 595-598.
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