Simultaneous determination of aminopyrine hydroxylation and aminopyrine N-demethylation in liver microsomes by high-performance liquid chromatography

Kazuhide Inoue, Kannosuke Fujimori, Keinosuke Mizokami, Momoko Sunouchi, Akira Takanaka, Yoshihito Omori

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Aminopyrine and its metabolites, including 3-hydroxymethyl-2-methyl-4-dimethylamino-1-phenyl-3-pyrazoline-5-one which is a hydroxylated metabolite of aminopyrine, were separated on a reversed-phase (C8) Radial-Pak column using a mobile phase of methanol-triethylamine-water (30:1:69) adjusted to pH 5.40 with acetic acid. Detection of the peak was performed by an ultraviolet detector at 254 nm. By the rapid and simple method, aminopyrine hydroxylation as well as aminopyrine N-demethylation in liver microsomes can be examined simultaneously.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume274
Issue numberC
DOIs
Publication statusPublished - 1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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