Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique

Xiao Lin Zhu; Toshiro Matsui; Keisuke Watanabe; Koso Shiraishi; Tatsuro Ueki; Kiyoshi Matsumoto

doi:10.1021/jf0720584

Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique

Xiao Lin Zhu, Toshiro Matsui, Keisuke Watanabe, Koso Shiraishi, Tatsuro Ueki, Kiyoshi Matsumoto

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 ± 0.037 mg/g, 2.50 ± 0.26 mg/g, and 0.096 ± 0.008 mg/g, respectively.

Original language	English
Pages (from-to)	8367-8371
Number of pages	5
Journal	Journal of Agricultural and Food Chemistry
Volume	55
Issue number	21
DOIs	https://doi.org/10.1021/jf0720584
Publication status	Published - Oct 17 2007

All Science Journal Classification (ASJC) codes

Chemistry(all)
Agricultural and Biological Sciences(all)

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Zhu, X. L., Matsui, T., Watanabe, K., Shiraishi, K., Ueki, T., & Matsumoto, K. (2007). Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique. Journal of Agricultural and Food Chemistry, 55(21), 8367-8371. https://doi.org/10.1021/jf0720584

Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique. / Zhu, Xiao Lin; Matsui, Toshiro; Watanabe, Keisuke et al.

In: Journal of Agricultural and Food Chemistry, Vol. 55, No. 21, 17.10.2007, p. 8367-8371.

Research output: Contribution to journal › Article › peer-review

Zhu, XL, Matsui, T, Watanabe, K, Shiraishi, K, Ueki, T & Matsumoto, K 2007, 'Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique', Journal of Agricultural and Food Chemistry, vol. 55, no. 21, pp. 8367-8371. https://doi.org/10.1021/jf0720584

Zhu XL, Matsui T, Watanabe K, Shiraishi K, Ueki T, Matsumoto K. Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique. Journal of Agricultural and Food Chemistry. 2007 Oct 17;55(21):8367-8371. doi: 10.1021/jf0720584

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abstract = "A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 ± 0.037 mg/g, 2.50 ± 0.26 mg/g, and 0.096 ± 0.008 mg/g, respectively.",

author = "Zhu, {Xiao Lin} and Toshiro Matsui and Keisuke Watanabe and Koso Shiraishi and Tatsuro Ueki and Kiyoshi Matsumoto",

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AU - Watanabe, Keisuke

AU - Shiraishi, Koso

AU - Ueki, Tatsuro

AU - Matsumoto, Kiyoshi

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N2 - A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 ± 0.037 mg/g, 2.50 ± 0.26 mg/g, and 0.096 ± 0.008 mg/g, respectively.

AB - A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 ± 0.037 mg/g, 2.50 ± 0.26 mg/g, and 0.096 ± 0.008 mg/g, respectively.

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Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique

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