Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique

Xiao Lin Zhu, Toshiro Matsui, Keisuke Watanabe, Koso Shiraishi, Tatsuro Ueki, Kiyoshi Matsumoto

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8 Citations (Scopus)

Abstract

A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 ± 0.037 mg/g, 2.50 ± 0.26 mg/g, and 0.096 ± 0.008 mg/g, respectively.

Original languageEnglish
Pages (from-to)8367-8371
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number21
DOIs
Publication statusPublished - Oct 17 2007

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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