Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique

Xiao Lin Zhu, Toshiro Matsui, Keisuke Watanabe, Koso Shiraishi, Tatsuro Ueki, Kiyoshi Matsumoto

Research output: Contribution to journalArticle

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Abstract

A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26% acetonitrile for Ala-Phe and Tyr-Pro (32% for Trp-Tyr) in 0.1% trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 ± 0.037 mg/g, 2.50 ± 0.26 mg/g, and 0.096 ± 0.008 mg/g, respectively.

Original languageEnglish
Pages (from-to)8367-8371
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number21
DOIs
Publication statusPublished - Oct 17 2007

Fingerprint

tryptophyltyrosine
tyrosyl-proline
Silanes
casein hydrolysates
peptidyl-dipeptidase A
High performance liquid chromatography
Peptidyl-Dipeptidase A
high performance liquid chromatography
heart
High Pressure Liquid Chromatography
peptides
Trifluoroacetic Acid
Peptides
antihypertensive agents
Antihypertensive Agents
Assays
sodium
acids
assays
methodology

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Simultaneous determination of angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique. / Zhu, Xiao Lin; Matsui, Toshiro; Watanabe, Keisuke; Shiraishi, Koso; Ueki, Tatsuro; Matsumoto, Kiyoshi.

In: Journal of Agricultural and Food Chemistry, Vol. 55, No. 21, 17.10.2007, p. 8367-8371.

Research output: Contribution to journalArticle

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abstract = "A replicate heart-cut column-switching HPLC method combined with two switching valves was newly developed for the simultaneous determination of three antihypertensive peptides (Ala-Phe, Tyr-Pro, and Trp-Tyr) in tryptic casein hydrolysate in one run-in assay. After a first separation on an octadecyl silane (ODS) column, heart-cuts of each peptide were individually separated on a subsequent analytical ODS column: 26{\%} acetonitrile for Ala-Phe and Tyr-Pro (32{\%} for Trp-Tyr) in 0.1{\%} trifluoroacetic acid containing 10 mM sodium 1-octanesulfonate at 0.8 mL/min. Ala-Phe, Tyr-Pro, and Trp-Tyr in casein hydrolysate were determined within 70 min to be 0.377 ± 0.037 mg/g, 2.50 ± 0.26 mg/g, and 0.096 ± 0.008 mg/g, respectively.",
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AU - Watanabe, Keisuke

AU - Shiraishi, Koso

AU - Ueki, Tatsuro

AU - Matsumoto, Kiyoshi

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