TY - JOUR
T1 - Simultaneous determination of bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry
AU - Kudo, Keiko
AU - Kiyoshima, Akiko
AU - Ohtsuka, Yukio
AU - Ikeda, Noriaki
PY - 2003/7/5
Y1 - 2003/7/5
N2 - We devised a sensitive and simple method to simultaneously determine bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. Bromvalerylurea and allylisopropylacetylurea were extracted using an Extrelut® column with an internal standard, 2-bromohexanoylurea, followed by derivatization with heptafluorobutyric anhydride. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of both compounds were linear in the concentration range from 0.01 to 10 μg/ml in both blood and urine samples. The lower limits of detection of bromvalerylurea and allylisopropylacetylurea were 0.005 and 0.005 μg/ml, respectively. This method proved most useful in accurately identifying these drugs in blood and urine from an autopsied individual.
AB - We devised a sensitive and simple method to simultaneously determine bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. Bromvalerylurea and allylisopropylacetylurea were extracted using an Extrelut® column with an internal standard, 2-bromohexanoylurea, followed by derivatization with heptafluorobutyric anhydride. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of both compounds were linear in the concentration range from 0.01 to 10 μg/ml in both blood and urine samples. The lower limits of detection of bromvalerylurea and allylisopropylacetylurea were 0.005 and 0.005 μg/ml, respectively. This method proved most useful in accurately identifying these drugs in blood and urine from an autopsied individual.
UR - http://www.scopus.com/inward/record.url?scp=0038359607&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038359607&partnerID=8YFLogxK
U2 - 10.1016/S1570-0232(03)00219-8
DO - 10.1016/S1570-0232(03)00219-8
M3 - Article
C2 - 12798177
AN - SCOPUS:0038359607
VL - 791
SP - 171
EP - 177
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1-2
ER -