Abstract
We devised a sensitive and simple method to simultaneously determine bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. Bromvalerylurea and allylisopropylacetylurea were extracted using an Extrelut® column with an internal standard, 2-bromohexanoylurea, followed by derivatization with heptafluorobutyric anhydride. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of both compounds were linear in the concentration range from 0.01 to 10 μg/ml in both blood and urine samples. The lower limits of detection of bromvalerylurea and allylisopropylacetylurea were 0.005 and 0.005 μg/ml, respectively. This method proved most useful in accurately identifying these drugs in blood and urine from an autopsied individual.
| Original language | English |
|---|---|
| Pages (from-to) | 171-177 |
| Number of pages | 7 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 791 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jul 5 2003 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Cell Biology
Cite this
Simultaneous determination of bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. / Kudo, Keiko; Kiyoshima, Akiko; Ohtsuka, Yukio; Ikeda, Noriaki.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 791, No. 1-2, 05.07.2003, p. 171-177.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Simultaneous determination of bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry
AU - Kudo, Keiko
AU - Kiyoshima, Akiko
AU - Ohtsuka, Yukio
AU - Ikeda, Noriaki
PY - 2003/7/5
Y1 - 2003/7/5
N2 - We devised a sensitive and simple method to simultaneously determine bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. Bromvalerylurea and allylisopropylacetylurea were extracted using an Extrelut® column with an internal standard, 2-bromohexanoylurea, followed by derivatization with heptafluorobutyric anhydride. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of both compounds were linear in the concentration range from 0.01 to 10 μg/ml in both blood and urine samples. The lower limits of detection of bromvalerylurea and allylisopropylacetylurea were 0.005 and 0.005 μg/ml, respectively. This method proved most useful in accurately identifying these drugs in blood and urine from an autopsied individual.
AB - We devised a sensitive and simple method to simultaneously determine bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. Bromvalerylurea and allylisopropylacetylurea were extracted using an Extrelut® column with an internal standard, 2-bromohexanoylurea, followed by derivatization with heptafluorobutyric anhydride. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of both compounds were linear in the concentration range from 0.01 to 10 μg/ml in both blood and urine samples. The lower limits of detection of bromvalerylurea and allylisopropylacetylurea were 0.005 and 0.005 μg/ml, respectively. This method proved most useful in accurately identifying these drugs in blood and urine from an autopsied individual.
UR - http://www.scopus.com/inward/record.url?scp=0038359607&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038359607&partnerID=8YFLogxK
U2 - 10.1016/S1570-0232(03)00219-8
DO - 10.1016/S1570-0232(03)00219-8
M3 - Article
VL - 791
SP - 171
EP - 177
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1-2
ER -