Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography-mass spectrometry

Shigetoshi Kage, Keiko Kudo, Hideaki Ikeda, Noriaki Ikeda

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26 Citations (Scopus)

Abstract

We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography-mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). The derivatives obtained were analyzed using gas chromatography-mass spectrometry in positive-ion electron ionization (EI) mode. The lower limit of detection for both compounds was 0.02 mM. The calibration curves for formate and acetate were linear over the concentration range from 0.05 to 5.0 mM. Accuracy and precision of the method were evaluated and the coefficients of variation were within 10%. With use of this method, levels of formate and acetate in whole blood can be determined in forensic cases.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume805
Issue number1
DOIs
Publication statusPublished - Jun 5 2004

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formic acid
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Acetates
Blood
Urine
Acetone
Calibration
Ionization
Limit of Detection
Buffers
Positive ions
Phosphates
Electrons
Ions
Derivatives

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "We devised a sensitive and simple method for simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography-mass spectrometry. Formate and acetate were alkylated with pentafluorobenzyl bromide in the mixture of acetone and phosphate buffer (pH 6.8). The derivatives obtained were analyzed using gas chromatography-mass spectrometry in positive-ion electron ionization (EI) mode. The lower limit of detection for both compounds was 0.02 mM. The calibration curves for formate and acetate were linear over the concentration range from 0.05 to 5.0 mM. Accuracy and precision of the method were evaluated and the coefficients of variation were within 10{\%}. With use of this method, levels of formate and acetate in whole blood can be determined in forensic cases.",
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