Simultaneous determination of four vasoactive phytochemicals in different pharmaceutical preparations by a simple HPLC-DAD method

Fawzia Ibrahim, Mohie Khaled Sharaf El-Din, Rania Nabih El-Shaheny, Asmaa Kamal El-Deen, Kuniyoshi Shimizu

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A new, simple isocratic HPLC method was developed and validated for the simultaneous estimation of four vasoactive phytochemicals: ascorbic acid (ASC), rutin (RUT), hesperidin (HSP), and diosmin (DSM) in different pharmaceutical preparations. Separation of the four compounds was achieved in about 8 min on a phenomenex-C18 column (250 mm x 4.6 mm, 5 μm particle size) using a mobile phase consists of 50 mM phosphate buffer (pH 4.0):acetonitrile (75:25, v/v) eluted at 1.0 mL min-1 with diode array detection at 275 nm. Linear correlation was obeyed over the concentration ranges of 2.0-60.0, 1.0-20.0, 2.0-50.0, and 5.0-100.0 μg mL-1 for ASC, RUT, HSP, and DSM, respectively. The developed method is efficient, reproducible and appropriate as a versatile method for quality control of various combinations of these drugs in different pharmaceutical preparations with high values of %recoveries which agreed well with the labeled claim. The method is also a stability-indicating one for DSM in its pure state and single-component tablets. A comparison of the results obtained by the developed method with those of the comparison methods confirmed no significant differences between them with respect to accuracy and precision.

Original languageEnglish
Pages (from-to)1858-1866
Number of pages9
JournalAnalytical Methods
Issue number8
Publication statusPublished - Feb 28 2016


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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