Simultaneous Determination of Ultratrace Prostaglandins in Biological Samples with High-Performance Liquid-Chromatography/Laser-Induced Fluorometry

S. Sakae, A. Harata, T. Kitamori, T. Sawada, A. Okubo, S. Toda, T. Shimizu

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A newly developed analytical technique to extract, derivatize, and simultaneously determine trace prostaglandins (PGs) in practical biological samples is described. Trace PGs were extracted by a column separation technique and derivatized by 9-anthryldiazomethane (ADAM). The conventional chemical procedure for determination of carboxylic acids using ADAM was reinvestigated to get separation of trace PGs from enormous amounts of coexisting biological substances and by- and decomposition products of ADAM. The PG derivatives were separated by high-performance liquid chromatography and detected by laser-induced fluorometry (HPLC/LIF). The minimum detectable absolute quantity of the HPLC/LIF system was 0.5 pg (1.3 fmol) per injection. For the overall chemical procedure, sub-nanogram per milliliter levels of PGs in human urine were determined simultaneously.

Original languageEnglish
Pages (from-to)355-361
Number of pages7
JournalMicrochemical Journal
Volume49
Issue number2-3
DOIs
Publication statusPublished - Apr 1 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy

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