A newly developed analytical technique to extract, derivatize, and simultaneously determine trace prostaglandins (PGs) in practical biological samples is described. Trace PGs were extracted by a column separation technique and derivatized by 9-anthryldiazomethane (ADAM). The conventional chemical procedure for determination of carboxylic acids using ADAM was reinvestigated to get separation of trace PGs from enormous amounts of coexisting biological substances and by- and decomposition products of ADAM. The PG derivatives were separated by high-performance liquid chromatography and detected by laser-induced fluorometry (HPLC/LIF). The minimum detectable absolute quantity of the HPLC/LIF system was 0.5 pg (1.3 fmol) per injection. For the overall chemical procedure, sub-nanogram per milliliter levels of PGs in human urine were determined simultaneously.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry