Simultaneous measurements of quantum yield and CO2 uptake for the assessment of non-assimilative electron flow in tree leaves

Michito Tsuyama, Hajime Kobayashi, Masaru Shinya, Hisashi Yahata, Yoshichika Kobayashi

Research output: Contribution to journalArticle

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Abstract

Using attached and detached leaves of Acer palmatum Thunb. and Rhaphiolepis umbellata Makino, pulse-modulated chlorophyll fluorescence and CO2 exchange were measured. Quantum yield of photosynthesis was determined from the fluorescence parameter (Fm′- Fs)/Fm′, where (Fm′- Fs) was defined as the difference between steady state chlorophyll fluorescence (Fs) and maximum fluorescence (Fm′) elicited by a saturating light pulse. The rate of electron transport through photosystem II (total electron flow) was calculated from the product of quantum yield and A(PFD), where A is the rate of absorbed photons as given by leaf absorptance, and PFD is the photon flux density at the leaf surface. The rate of electron transport dependant on CO2 uptake (assimilative electron flow) was calculated from the gross photosynthetic rate in a leaf. The difference between the rates of total and assimilative electron transport was denoted as the rate of non-assimilative electron transport which depends on photorespiration and oxygen reduction. Available data provided quantitative information on the rate of non-assimilative electron flow in intact leaves. When leaf photosynthesis of A. palmatum was measured under sunlight, the rates of total and assimilative electron transport were determined to be approximately 900 and 150 μmol equiv. e/mg Chl · h, respectively. The difference (750 μmol equiv. e/mg Chl · h) was attributed to the activity of non-assimilative electron flow. The ratio of total to assimilative electron flow was found to increase gradually with rising in irradiance. The results suggest that non-assimilative electron flow occurred at much higher rate than assimilative electron flow at high irradiance. Implications of the results are briefly discussed in relation to photosynthesis limitation in tree leaves.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalJournal of Forest Research
Volume1
Issue number2
DOIs
Publication statusPublished - 1996

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electrons
uptake mechanisms
electron transfer
electron
fluorescence
Acer palmatum
leaves
photosynthesis
Rhaphiolepis
chlorophyll
photorespiration
irradiance
photosystem II
solar radiation
rate
photon flux density
oxygen
photons

All Science Journal Classification (ASJC) codes

  • Forestry

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Simultaneous measurements of quantum yield and CO2 uptake for the assessment of non-assimilative electron flow in tree leaves. / Tsuyama, Michito; Kobayashi, Hajime; Shinya, Masaru; Yahata, Hisashi; Kobayashi, Yoshichika.

In: Journal of Forest Research, Vol. 1, No. 2, 1996, p. 79-85.

Research output: Contribution to journalArticle

Tsuyama, Michito ; Kobayashi, Hajime ; Shinya, Masaru ; Yahata, Hisashi ; Kobayashi, Yoshichika. / Simultaneous measurements of quantum yield and CO2 uptake for the assessment of non-assimilative electron flow in tree leaves. In: Journal of Forest Research. 1996 ; Vol. 1, No. 2. pp. 79-85.
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