Simultaneous profiling of 17 steroid hormones for the evaluation of endocrine-disrupting chemicals in H295R cells

Udi Jumhawan, Toshiyuki Yamashita, Kazuya Ishida, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: There is urgent need to develop a new protocol for the evaluation of chemical substances to potentially interact with the endocrine system and induce numerous pathological issues. The recently validated in vitro screening assay is limited on monitoring two steroid hormones. Methodology & results: The H295R model cell was exposed to seven endocrine disrupting chemicals (EDCs). The levels of 17 steroid hormones in cell extracts were subsequently determined by a quantitative targeted GC/MS/MS method. Through wide coverage, this system managed to capture the effects of exposure to increasing EDCs concentrations in the entire steroidogenic pathways. Conclusion: The developed approach could be beneficial for the mechanistic investigation of EDCs.

Original languageEnglish
Pages (from-to)67-79
Number of pages13
JournalBioanalysis
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 2017

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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