Simultaneous quantification of lignans in Arabidopsis thaliana by highly sensitive capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry

Atsushi Okazawa, Katsuhito Hori, Yohei Okumura, Yoshihiro Izumi, Naoki Hata, Takeshi Bamba, Eiichiro Fukusaki, Eiichiro Ono, Honoo Satake, Akio Kobayashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lignans are phenylpropanoid dimers in which the monomers are linked by the central carbon (C8) atoms. Because many lignans have physiological effects, including antioxidant activity, they are now in demand as components in health foods. However, the lignan biosynthetic pathways in plants are only now being understood. Recently, lariciresinol was detected in Arabidopsis thaliana. This observation indicated the existence of common lignan biosynthetic pathways in A. thaliana, despite a low amount of lignans other than lariciresinol glycosides. In this study, we established a highly sensitive analytical method that enables quantification of both glycoside and aglycone forms of lignans in A. thaliana simultaneously using capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry. Some lignans not previously detected in A. thaliana were quantified in extracts of both roots and shoots. Our method can be used for the comprehensive analysis of lignans in small samples from mutants and transformants. This method will be utilized to elucidate the metabolic pathways and physiological roles of lignans as well as the regulation of their biosynthesis in plants.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalPlant Biotechnology
Volume28
Issue number3
DOIs
Publication statusPublished - Aug 18 2011
Externally publishedYes

Fingerprint

Lignans
lignans
Arabidopsis
Liquid Chromatography
ionization
liquid chromatography
Mass Spectrometry
Arabidopsis thaliana
traps
mass spectrometry
Ions
ions
biochemical pathways
Biosynthetic Pathways
glycosides
health foods
phenylpropanoids
Glycosides
Metabolic Networks and Pathways
analytical methods

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Simultaneous quantification of lignans in Arabidopsis thaliana by highly sensitive capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry. / Okazawa, Atsushi; Hori, Katsuhito; Okumura, Yohei; Izumi, Yoshihiro; Hata, Naoki; Bamba, Takeshi; Fukusaki, Eiichiro; Ono, Eiichiro; Satake, Honoo; Kobayashi, Akio.

In: Plant Biotechnology, Vol. 28, No. 3, 18.08.2011, p. 287-293.

Research output: Contribution to journalArticle

Okazawa, Atsushi ; Hori, Katsuhito ; Okumura, Yohei ; Izumi, Yoshihiro ; Hata, Naoki ; Bamba, Takeshi ; Fukusaki, Eiichiro ; Ono, Eiichiro ; Satake, Honoo ; Kobayashi, Akio. / Simultaneous quantification of lignans in Arabidopsis thaliana by highly sensitive capillary liquid chromatography-electrospray ionization-ion trap mass spectrometry. In: Plant Biotechnology. 2011 ; Vol. 28, No. 3. pp. 287-293.
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