Simultaneous separation, metering and dilution of plasma from human whole blood using a microchip with an interchannel microstructure

Tomoya Tachi, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this research, we designed and operated a microchip with an interchannel microstructure for separation of plasma from human whole blood and for metering and diluting the plasma. The plasma separation was based on both cross-flow filtration and sedimentation of red blood cells in the microchannels. Metering and diluting the plasma was based on volume control of liquid in the microchannels by syringe pumps. Simultaneous operations to separate, meter and dilute plasma obtained from whole blood were done in microchannels using this microchip, and plasma precisely diluted by a factor of 6 was generated without hemolysis in the microchannels in about 3 minutes. This microchip is easily connected with other microchannels and microfluidic devices for quantitative analyses.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages427-429
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - Jan 1 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Fingerprint

Dilution
Microchannels
Blood
Plasmas
Microstructure
Syringes
Sedimentation
Microfluidics
Cells
Pumps
Liquids

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Tachi, T., Kaji, N., Okamoto, Y., Tokeshi, M., & Baba, Y. (2009). Simultaneous separation, metering and dilution of plasma from human whole blood using a microchip with an interchannel microstructure. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 427-429). Chemical and Biological Microsystems Society.

Simultaneous separation, metering and dilution of plasma from human whole blood using a microchip with an interchannel microstructure. / Tachi, Tomoya; Kaji, Noritada; Okamoto, Yukihiro; Tokeshi, Manabu; Baba, Yoshinobu.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 427-429.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tachi, T, Kaji, N, Okamoto, Y, Tokeshi, M & Baba, Y 2009, Simultaneous separation, metering and dilution of plasma from human whole blood using a microchip with an interchannel microstructure. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 427-429, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 11/1/09.
Tachi T, Kaji N, Okamoto Y, Tokeshi M, Baba Y. Simultaneous separation, metering and dilution of plasma from human whole blood using a microchip with an interchannel microstructure. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 427-429
Tachi, Tomoya ; Kaji, Noritada ; Okamoto, Yukihiro ; Tokeshi, Manabu ; Baba, Yoshinobu. / Simultaneous separation, metering and dilution of plasma from human whole blood using a microchip with an interchannel microstructure. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 427-429
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