Simultaneous separation, metering, and dilution of plasma from human whole blood in a microfluidic system

Tomoya Tachi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

In about a 3 min period, we have simultaneously separated plasma from human whole blood and metered and diluted the plasma using a microchip with an interchannel microstructure. The plasma separation was based on both cross-flow filtration and sedimentation of red blood cells in the microchannels. Metering and diluting operations of the plasma were based on volume control of liquid in the microchannels by syringe pumps. On this microchip, we produced plasma diluted by a factor of 6 from whole blood containing theophylline and we observed very little hemolysis. It is possible to separate plasma from one or just several drops of whole blood by using this microchip.

Original languageEnglish
Pages (from-to)3194-3198
Number of pages5
JournalAnalytical Chemistry
Volume81
Issue number8
DOIs
Publication statusPublished - Apr 15 2009
Externally publishedYes

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Microfluidics
Dilution
Blood
Plasmas
Microchannels
Syringes
Theophylline
Sedimentation
Cells
Pumps
Microstructure
Liquids

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Simultaneous separation, metering, and dilution of plasma from human whole blood in a microfluidic system. / Tachi, Tomoya; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

In: Analytical Chemistry, Vol. 81, No. 8, 15.04.2009, p. 3194-3198.

Research output: Contribution to journalArticle

Tachi, Tomoya ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Simultaneous separation, metering, and dilution of plasma from human whole blood in a microfluidic system. In: Analytical Chemistry. 2009 ; Vol. 81, No. 8. pp. 3194-3198.
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