Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem

Jun Shigeto, Yoshitaka Itoh, Sakie Hirao, Kaori Ohira, Koki Fujita, Yuji Tsutsumi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-DNA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol-β-aryl (β-O-4) linkage type, especially β-O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants exhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved cell wall degradability. This study will contribute to progress in lignin bioengineering to improve lignocellulosic biomass.

Original languageEnglish
Pages (from-to)349-356
Number of pages8
JournalJournal of Integrative Plant Biology
Volume57
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

Fingerprint

Lignin
Arabidopsis
lignin
stems
Peroxidases
Polymerization
peroxidases
polymerization
mutants
Bioengineering
Growth
Heme
Biomass
Cell Wall
bioengineering
Infertility
tissue analysis
dwarfing
lignification
derivatization

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

Cite this

Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem. / Shigeto, Jun; Itoh, Yoshitaka; Hirao, Sakie; Ohira, Kaori; Fujita, Koki; Tsutsumi, Yuji.

In: Journal of Integrative Plant Biology, Vol. 57, No. 4, 01.04.2015, p. 349-356.

Research output: Contribution to journalArticle

@article{c9c0dae8b7524acc9f98fc45b00fa956,
title = "Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem",
abstract = "Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-DNA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol-β-aryl (β-O-4) linkage type, especially β-O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants exhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved cell wall degradability. This study will contribute to progress in lignin bioengineering to improve lignocellulosic biomass.",
author = "Jun Shigeto and Yoshitaka Itoh and Sakie Hirao and Kaori Ohira and Koki Fujita and Yuji Tsutsumi",
year = "2015",
month = "4",
day = "1",
doi = "10.1111/jipb.12334",
language = "English",
volume = "57",
pages = "349--356",
journal = "Journal of Integrative Plant Biology",
issn = "1672-9072",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Simultaneously disrupting AtPrx2, AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem

AU - Shigeto, Jun

AU - Itoh, Yoshitaka

AU - Hirao, Sakie

AU - Ohira, Kaori

AU - Fujita, Koki

AU - Tsutsumi, Yuji

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-DNA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol-β-aryl (β-O-4) linkage type, especially β-O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants exhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved cell wall degradability. This study will contribute to progress in lignin bioengineering to improve lignocellulosic biomass.

AB - Plant class III heme peroxidases catalyze lignin polymerization. Previous reports have shown that at least three Arabidopsis thaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, are involved in stem lignification using T-DNA insertion mutants, atprx2, atprx25, and atprx71. Here, we generated three double mutants, atprx2/atprx25, atprx2/atprx71, and atprx25/atprx71, and investigated the impact of the simultaneous deficiency of these peroxidases on lignins and plant growth. Stem tissue analysis using the acetyl bromide method and derivatization followed by reductive cleavage revealed improved lignin characteristics, such as lowered lignin content and increased arylglycerol-β-aryl (β-O-4) linkage type, especially β-O-4 linked syringyl units, in lignin, supporting the roles of these genes in lignin polymerization. In addition, none of the double mutants exhibited severe growth defects, such as shorter plant stature, dwarfing, or sterility, and their stems had improved cell wall degradability. This study will contribute to progress in lignin bioengineering to improve lignocellulosic biomass.

UR - http://www.scopus.com/inward/record.url?scp=84926180956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926180956&partnerID=8YFLogxK

U2 - 10.1111/jipb.12334

DO - 10.1111/jipb.12334

M3 - Article

VL - 57

SP - 349

EP - 356

JO - Journal of Integrative Plant Biology

JF - Journal of Integrative Plant Biology

SN - 1672-9072

IS - 4

ER -