Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants

Waraporn Putalun, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding.

Original languageEnglish
Title of host publicationApplications of Plant Metabolic Engineering
PublisherSpringer Netherlands
Pages283-295
Number of pages13
Volume9781402060311
ISBN (Electronic)9781402060311
ISBN (Print)9781402060304
DOIs
Publication statusPublished - Jan 1 2007

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

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    Putalun, W., Tanaka, H., & Shoyama, Y. (2007). Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. In Applications of Plant Metabolic Engineering (Vol. 9781402060311, pp. 283-295). Springer Netherlands. https://doi.org/10.1007/978-1-4020-6031-1_12