Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants

Waraporn Putalun, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding.

Original languageEnglish
Title of host publicationApplications of Plant Metabolic Engineering
PublisherSpringer Netherlands
Pages283-295
Number of pages13
Volume9781402060311
ISBN (Electronic)9781402060311
ISBN (Print)9781402060304
DOIs
Publication statusPublished - Jan 1 2007
Externally publishedYes

Fingerprint

Single-Chain Antibodies
Solanum aculeatissimum
secondary metabolites
genetically modified organisms
biosynthesis
antibodies
glycosides
recombinant antibodies
antigens
breeding methods
Glycosides
plant breeding
monoclonal antibodies
proteins
Escherichia coli
Solanum
Antigens
Immunoglobulin Fragments
breeding
Breeding

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Putalun, W., Tanaka, H., & Shoyama, Y. (2007). Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. In Applications of Plant Metabolic Engineering (Vol. 9781402060311, pp. 283-295). Springer Netherlands. https://doi.org/10.1007/978-1-4020-6031-1_12

Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. / Putalun, Waraporn; Tanaka, Hiroyuki; Shoyama, Yukihiro.

Applications of Plant Metabolic Engineering. Vol. 9781402060311 Springer Netherlands, 2007. p. 283-295.

Research output: Chapter in Book/Report/Conference proceedingChapter

Putalun, W, Tanaka, H & Shoyama, Y 2007, Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. in Applications of Plant Metabolic Engineering. vol. 9781402060311, Springer Netherlands, pp. 283-295. https://doi.org/10.1007/978-1-4020-6031-1_12
Putalun W, Tanaka H, Shoyama Y. Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. In Applications of Plant Metabolic Engineering. Vol. 9781402060311. Springer Netherlands. 2007. p. 283-295 https://doi.org/10.1007/978-1-4020-6031-1_12
Putalun, Waraporn ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants. Applications of Plant Metabolic Engineering. Vol. 9781402060311 Springer Netherlands, 2007. pp. 283-295
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