Single DNA molecule extraction from single bacterium using nanowire structures

Kouhei Ootsuka, Takao Yasui, Noritada Kaji, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

There are many methods to extract cellular DNA molecule. But these methods need troublesome procedures such as adding lysis reagent and heating samples. However, nanowire embedded microchannel can extract DNA molecule only by injecting cell sample and applying electric field. This nanowire has needle-like shaped and can damage cellular membrane because of physical interaction between the nanowire s and the cellular membrane. We damaged cell and extracted DNA molecules from the damaged-membrane by applying electric field.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages479-481
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Nanowires
Bacteria
DNA
Membranes
Molecules
Electric fields
Microchannels
Needles
Heating

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Ootsuka, K., Yasui, T., Kaji, N., Rahong, S., Yanagida, T., Kanai, M., ... Baba, Y. (2013). Single DNA molecule extraction from single bacterium using nanowire structures. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 479-481). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.

Single DNA molecule extraction from single bacterium using nanowire structures. / Ootsuka, Kouhei; Yasui, Takao; Kaji, Noritada; Rahong, Sakon; Yanagida, Takeshi; Kanai, Masaki; Nagashima, Kazuki; Kawai, Tomoji; Baba, Yoshinobu.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 479-481 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ootsuka, K, Yasui, T, Kaji, N, Rahong, S, Yanagida, T, Kanai, M, Nagashima, K, Kawai, T & Baba, Y 2013, Single DNA molecule extraction from single bacterium using nanowire structures. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 1, Chemical and Biological Microsystems Society, pp. 479-481, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Ootsuka K, Yasui T, Kaji N, Rahong S, Yanagida T, Kanai M et al. Single DNA molecule extraction from single bacterium using nanowire structures. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 479-481. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
Ootsuka, Kouhei ; Yasui, Takao ; Kaji, Noritada ; Rahong, Sakon ; Yanagida, Takeshi ; Kanai, Masaki ; Nagashima, Kazuki ; Kawai, Tomoji ; Baba, Yoshinobu. / Single DNA molecule extraction from single bacterium using nanowire structures. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 479-481 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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