Single DNA/protein interaction analysis using quantum dot conjugates and microfluidic systems

D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We have designed a microfluidic detection platform in which we can monitor and analyze single DNA/protein dynamic interaction using “single-molecule fluorescence tracing”. The use of quantum dot (QD) labelling on DNA, polydimethylsiloxane (PDMS) microchip, micro patterning of active enzymes, and total internal reflection fluorescence (TIRF) microscope was successfully applied for nano-space experiments in micrometer-sized channels. We used this detection platform to observe the enzymatic reaction of restriction endonuclease (EcoRI) and measured the duration of its cleavage reaction.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages1619-1621
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: Oct 7 2007Oct 11 2007

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
CountryFrance
CityParis
Period10/7/0710/11/07

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Onoshima, D., Kaji, N., Tokeshi, M., & Baba, Y. (2007). Single DNA/protein interaction analysis using quantum dot conjugates and microfluidic systems. In J-L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 (pp. 1619-1621). (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007). Chemical and Biological Microsystems Society.