Single long DNA molecule analysis using fluorescence microscopy.

N. Kaji, M. Ueda, Y. Baba

Research output: Contribution to conferencePaperpeer-review

1 Citation (Scopus)


Single long DNA molecule (T4 DNA) in agarose gel was visualized with a fluorescence microscope. We confirmed alternating current electric fields is effective for stretching of single DNA molecule in agarose gel. This stretching phenomenon was observed with wide range of agarose gel concentration from 0.5%(W/V) to 1.5%. From this observation, the presence of agarose gel fiber is essential for this stretching phenomenon. The stretching process of several DNA molecules in gel shows discontinuity, which is never observed in polymer systems. It would be based on topological restriction from gel fibers.

Original languageEnglish
Number of pages2
Publication statusPublished - Jan 1 2000
Externally publishedYes


Dive into the research topics of 'Single long DNA molecule analysis using fluorescence microscopy.'. Together they form a unique fingerprint.

Cite this