Single long DNA molecule analysis using fluorescence microscopy.

N. Kaji, M. Ueda, Y. Baba

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

Single long DNA molecule (T4 DNA) in agarose gel was visualized with a fluorescence microscope. We confirmed alternating current electric fields is effective for stretching of single DNA molecule in agarose gel. This stretching phenomenon was observed with wide range of agarose gel concentration from 0.5%(W/V) to 1.5%. From this observation, the presence of agarose gel fiber is essential for this stretching phenomenon. The stretching process of several DNA molecules in gel shows discontinuity, which is never observed in polymer systems. It would be based on topological restriction from gel fibers.

Original languageEnglish
Pages247-248
Number of pages2
Publication statusPublished - Jan 1 2000
Externally publishedYes

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