Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion

Michihiko Nakano, Naohito Nakai, Hirofumi Kurita, Jun Komatsu, Kazunori Takashima, Shinji Katsura, Akira Mizuno

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.

Original languageEnglish
Pages (from-to)293-295
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume99
Issue number3
DOIs
Publication statusPublished - Jan 1 2005
Externally publishedYes

Fingerprint

Polymerase chain reaction
Transcription
Emulsions
Reverse Transcription
Oils
Polymerase Chain Reaction
Molecules
Water
RNA
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion. / Nakano, Michihiko; Nakai, Naohito; Kurita, Hirofumi; Komatsu, Jun; Takashima, Kazunori; Katsura, Shinji; Mizuno, Akira.

In: Journal of Bioscience and Bioengineering, Vol. 99, No. 3, 01.01.2005, p. 293-295.

Research output: Contribution to journalArticle

Nakano, Michihiko ; Nakai, Naohito ; Kurita, Hirofumi ; Komatsu, Jun ; Takashima, Kazunori ; Katsura, Shinji ; Mizuno, Akira. / Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion. In: Journal of Bioscience and Bioengineering. 2005 ; Vol. 99, No. 3. pp. 293-295.
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