Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion

Michihiko Nakano, Naohito Nakai, Hirofumi Kurita, Jun Komatsu, Kazunori Takashima, Shinji Katsura, Akira Mizuno

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.

Original languageEnglish
Pages (from-to)293-295
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume99
Issue number3
DOIs
Publication statusPublished - Mar 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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