Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies

Judy E. Anderson, Ashley C. Wozniak, Wataru Mizunoya

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Citations (Scopus)

Abstract

The technique of single muscle-fiber cultures has already proven valuable in extending knowledge of myogenesis, stem cell heterogeneity, the stem cell niche in skeletal muscle, and satellite cell activation. This report reviews the background of the model and applications, and details the procedures of muscle dissection, fiber digestion and isolation, cleaning the fiber preparation, plating fibers, and extensions of the technique for studying activation from stable quiescence of satellite cells, mRNA expression by in situ hybridization and regulation of satellite cell activation in zebrafish muscle by nitric oxide, hepatocyte growth factor.

Original languageEnglish
Title of host publicationMyogenesis
Subtitle of host publicationMethods and Protocols
EditorsJoseph DiMario
Pages85-102
Number of pages18
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume798
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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