Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses

S. Baba, Y. Kukita, K. Higasa, T. Tahira, K. Hayashi

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

We describe a new environment of a single-stranded conformational polymorphism (SSCP) analysis using automated capillary array sequencers (e.g., ABI PRISm® 3100 and 3700). In this environment, electrophoretic conditions, settings for instrument management, and software for data analysis are adjusted for SSCP analysis. Highly reproducible results are obtained with this new system, and fragments with mutations and/or polymorphisms in different capillaries or different runs can be reliably detected. The relative peak heights between alleles are quantitative and reproducible between runs, and so allele frequencies of single nucleotide polymorphisms can be accurately estimated by a pooled DNA strategy. The method allows unattended, low-cost, and quantitative SSCP analysis using instruments that are widely accessible.

Original languageEnglish
Pages (from-to)746-750
Number of pages5
JournalBioTechniques
Volume34
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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