Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses

Shingo Baba, Y. Kukita, K. Higasa, T. Tahira, K. Hayashi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We describe a new environment of a single-stranded conformational polymorphism (SSCP) analysis using automated capillary array sequencers (e.g., ABI PRISm® 3100 and 3700). In this environment, electrophoretic conditions, settings for instrument management, and software for data analysis are adjusted for SSCP analysis. Highly reproducible results are obtained with this new system, and fragments with mutations and/or polymorphisms in different capillaries or different runs can be reliably detected. The relative peak heights between alleles are quantitative and reproducible between runs, and so allele frequencies of single nucleotide polymorphisms can be accurately estimated by a pooled DNA strategy. The method allows unattended, low-cost, and quantitative SSCP analysis using instruments that are widely accessible.

Original languageEnglish
Pages (from-to)746-750
Number of pages5
JournalBioTechniques
Volume34
Issue number4
Publication statusPublished - Apr 1 2003

Fingerprint

Single-Stranded Conformational Polymorphism
Capillary Electrophoresis
Electrophoresis
Polymorphism
Gene Frequency
Single Nucleotide Polymorphism
Software
Alleles
Costs and Cost Analysis
Mutation
DNA
Nucleotides
Costs

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses. / Baba, Shingo; Kukita, Y.; Higasa, K.; Tahira, T.; Hayashi, K.

In: BioTechniques, Vol. 34, No. 4, 01.04.2003, p. 746-750.

Research output: Contribution to journalArticle

Baba, Shingo ; Kukita, Y. ; Higasa, K. ; Tahira, T. ; Hayashi, K. / Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses. In: BioTechniques. 2003 ; Vol. 34, No. 4. pp. 746-750.
@article{562893bd84a546c3b498b695eaac1053,
title = "Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses",
abstract = "We describe a new environment of a single-stranded conformational polymorphism (SSCP) analysis using automated capillary array sequencers (e.g., ABI PRISm{\circledR} 3100 and 3700). In this environment, electrophoretic conditions, settings for instrument management, and software for data analysis are adjusted for SSCP analysis. Highly reproducible results are obtained with this new system, and fragments with mutations and/or polymorphisms in different capillaries or different runs can be reliably detected. The relative peak heights between alleles are quantitative and reproducible between runs, and so allele frequencies of single nucleotide polymorphisms can be accurately estimated by a pooled DNA strategy. The method allows unattended, low-cost, and quantitative SSCP analysis using instruments that are widely accessible.",
author = "Shingo Baba and Y. Kukita and K. Higasa and T. Tahira and K. Hayashi",
year = "2003",
month = "4",
day = "1",
language = "English",
volume = "34",
pages = "746--750",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Eaton Publishing Company",
number = "4",

}

TY - JOUR

T1 - Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses

AU - Baba, Shingo

AU - Kukita, Y.

AU - Higasa, K.

AU - Tahira, T.

AU - Hayashi, K.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - We describe a new environment of a single-stranded conformational polymorphism (SSCP) analysis using automated capillary array sequencers (e.g., ABI PRISm® 3100 and 3700). In this environment, electrophoretic conditions, settings for instrument management, and software for data analysis are adjusted for SSCP analysis. Highly reproducible results are obtained with this new system, and fragments with mutations and/or polymorphisms in different capillaries or different runs can be reliably detected. The relative peak heights between alleles are quantitative and reproducible between runs, and so allele frequencies of single nucleotide polymorphisms can be accurately estimated by a pooled DNA strategy. The method allows unattended, low-cost, and quantitative SSCP analysis using instruments that are widely accessible.

AB - We describe a new environment of a single-stranded conformational polymorphism (SSCP) analysis using automated capillary array sequencers (e.g., ABI PRISm® 3100 and 3700). In this environment, electrophoretic conditions, settings for instrument management, and software for data analysis are adjusted for SSCP analysis. Highly reproducible results are obtained with this new system, and fragments with mutations and/or polymorphisms in different capillaries or different runs can be reliably detected. The relative peak heights between alleles are quantitative and reproducible between runs, and so allele frequencies of single nucleotide polymorphisms can be accurately estimated by a pooled DNA strategy. The method allows unattended, low-cost, and quantitative SSCP analysis using instruments that are widely accessible.

UR - http://www.scopus.com/inward/record.url?scp=0037390015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037390015&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 746

EP - 750

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 4

ER -