Site restricted and neuron dominant expression of α2,8sialyltansferase gene in the adult mouse brain and retina

Akihito Yamamoto, Shuji Yamashiro, Satoshi Fukumoto, Masashi Haraguchi, Mitsuru Atsuta, Hiroshi Shiku, Koichi Furukawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Gene expression of the α2,8sialyltransferase (α2,8S-T) responsible for GD3 synthesis in the adult mouse brain and retina was analysed by reverse transcription-polymerase chain reaction/Southern blotting (RT-PCR/Southern) and in situ hybridization. Among various portions of the brain, high levels of 9.5 kb mRNA were observed in the retina and midbrain. Results of RT-PCR/Southern did not necessarily correlate with the enzyme activities in the individual sites. In situ hybridization analysis revealed that this gene was characteristically expressed in the inner segment of photoreceptor cells, some nuclei in the midbrain, cranial nerve nuclei in the pons-medulla, Purkinje cells in the cerebellum, pyramidal cells of the hippocampus and granular cells of the dentate gyrus. In the retina, the α2,8S-T gene was broadly expressed over the layers during development, and retained high expression levels in the photoreceptor cells of adult mice consistent with high expression of GD3. Destruction of neurons in the hippocampus and dentate gyrus by injection of kainic acid and colchicine respectively resulted in the disappearance of the hybridization signal, suggesting that the α2,8S-T gene was mainly expressed by neurons.

Original languageEnglish
Pages (from-to)471-480
Number of pages10
JournalGlycoconjugate Journal
Volume13
Issue number3
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Neurons
Retina
Brain
Photoreceptor Cells
Genes
Polymerase chain reaction
Dentate Gyrus
Transcription
Mesencephalon
Southern Blotting
Reverse Transcription
In Situ Hybridization
Hippocampus
Polymerase Chain Reaction
Pons
Cranial Nerves
Pyramidal Cells
Kainic Acid
Purkinje Cells
Colchicine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Site restricted and neuron dominant expression of α2,8sialyltansferase gene in the adult mouse brain and retina. / Yamamoto, Akihito; Yamashiro, Shuji; Fukumoto, Satoshi; Haraguchi, Masashi; Atsuta, Mitsuru; Shiku, Hiroshi; Furukawa, Koichi.

In: Glycoconjugate Journal, Vol. 13, No. 3, 01.01.1996, p. 471-480.

Research output: Contribution to journalArticle

Yamamoto, Akihito ; Yamashiro, Shuji ; Fukumoto, Satoshi ; Haraguchi, Masashi ; Atsuta, Mitsuru ; Shiku, Hiroshi ; Furukawa, Koichi. / Site restricted and neuron dominant expression of α2,8sialyltansferase gene in the adult mouse brain and retina. In: Glycoconjugate Journal. 1996 ; Vol. 13, No. 3. pp. 471-480.
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