Site-specific carbon isotope analysis of aromatic carboxylic acids by elemental analysis/pyrolysis/isotope ratio mass spectrometry

Yasuhiro Oba, Hiroshi Naraoka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Site-specific carbon isotope composition of organic compounds can provide useful information on their origin and history in natural environments. Site-specific isotope analyses of small amounts of organic compounds (sub-nanomolar level), such as short-chain carboxylic acids and amino acid analogues, have been performed using gas chromatography/pyrolysis/isotope ratio mass spectrometry (GC/pyrolysis/IRMS). These analyses were previously limited to volatile compounds. In this study, site-specific carbon isotope analysis has been developed for non-volatile aromatic carboxylic acids at sub-micromolar level by decarboxylation using a continuous flow elemental analysis (EA)/ pyrolysis/IRMS technique. Benzoic acid, 2-naphthylacetic acid and 1-pyrenecarboxylic acid were pyrolyzed at 500-1000°C by EA/pyrolysis/IRMS to produce CO2 for δ13C measurement of the carboxyl group. These three aromatic acids were most efficiently pyrolyzed at 750°C. Conventional sealed-tube pyrolysis was also conducted for comparison. The δ13C values of CO2 generated by the continuous flow technique were within 1.0‰ of those performed by the conventional technique, indicating that the new continuous flow technique can accurately analyze the carbon isotopic composition of the carboxyl group in aromatic carboxylic acids. The new continuous flow technique is simple, rapid and uses small sample sizes, so this technique will be useful for characterizing the isotopic signature of carboxyl groups in organic compounds.

Original languageEnglish
Pages (from-to)3649-3653
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume20
Issue number24
DOIs
Publication statusPublished - Dec 22 2006

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Carbon Isotopes
Carboxylic Acids
Carboxylic acids
Isotopes
Mass spectrometry
Pyrolysis
Organic compounds
Chemical analysis
Benzoic Acid
Gas chromatography
Carbon
Amino Acids

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Cite this

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abstract = "Site-specific carbon isotope composition of organic compounds can provide useful information on their origin and history in natural environments. Site-specific isotope analyses of small amounts of organic compounds (sub-nanomolar level), such as short-chain carboxylic acids and amino acid analogues, have been performed using gas chromatography/pyrolysis/isotope ratio mass spectrometry (GC/pyrolysis/IRMS). These analyses were previously limited to volatile compounds. In this study, site-specific carbon isotope analysis has been developed for non-volatile aromatic carboxylic acids at sub-micromolar level by decarboxylation using a continuous flow elemental analysis (EA)/ pyrolysis/IRMS technique. Benzoic acid, 2-naphthylacetic acid and 1-pyrenecarboxylic acid were pyrolyzed at 500-1000°C by EA/pyrolysis/IRMS to produce CO2 for δ13C measurement of the carboxyl group. These three aromatic acids were most efficiently pyrolyzed at 750°C. Conventional sealed-tube pyrolysis was also conducted for comparison. The δ13C values of CO2 generated by the continuous flow technique were within 1.0‰ of those performed by the conventional technique, indicating that the new continuous flow technique can accurately analyze the carbon isotopic composition of the carboxyl group in aromatic carboxylic acids. The new continuous flow technique is simple, rapid and uses small sample sizes, so this technique will be useful for characterizing the isotopic signature of carboxyl groups in organic compounds.",
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