Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

Shohei Uchinomiya, Hiroshi Nonaka, Sho Hei Fujishima, Shinya Tsukiji, Akio Ojida, Itaru Hamachi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.

Original languageEnglish
Pages (from-to)5880-5882
Number of pages3
JournalChemical Communications
Issue number39
DOIs
Publication statusPublished - Oct 12 2009

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Labeling
Proteins
Histidine

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe. / Uchinomiya, Shohei; Nonaka, Hiroshi; Fujishima, Sho Hei; Tsukiji, Shinya; Ojida, Akio; Hamachi, Itaru.

In: Chemical Communications, No. 39, 12.10.2009, p. 5880-5882.

Research output: Contribution to journalArticle

Uchinomiya, Shohei ; Nonaka, Hiroshi ; Fujishima, Sho Hei ; Tsukiji, Shinya ; Ojida, Akio ; Hamachi, Itaru. / Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe. In: Chemical Communications. 2009 ; No. 39. pp. 5880-5882.
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