Abstract
A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.
| Original language | English |
|---|---|
| Pages (from-to) | 5880-5882 |
| Number of pages | 3 |
| Journal | Chemical Communications |
| Issue number | 39 |
| DOIs | |
| Publication status | Published - Jan 1 2009 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Catalysis
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Chemistry(all)
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry
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Cite this
Uchinomiya, S. H., Nonaka, H., Fujishima, S. H., Tsukiji, S., Ojida, A., & Hamachi, I. (2009). Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe. Chemical Communications, (39), 5880-5882. https://doi.org/10.1039/b912025d