Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

Sho Hei Uchinomiya; Hiroshi Nonaka; Sho Hei Fujishima; Shinya Tsukiji; Akio Ojida; Itaru Hamachi

doi:10.1039/b912025d

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

Sho Hei Uchinomiya, Hiroshi Nonaka, Sho Hei Fujishima, Shinya Tsukiji, Akio Ojida, Itaru Hamachi

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.

Original language	English
Pages (from-to)	5880-5882
Number of pages	3
Journal	Chemical Communications
Issue number	39
DOIs	https://doi.org/10.1039/b912025d
Publication status	Published - Jan 1 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/b912025d

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AU - Uchinomiya, Sho Hei

AU - Nonaka, Hiroshi

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AU - Tsukiji, Shinya

AU - Ojida, Akio

AU - Hamachi, Itaru

PY - 2009/1/1

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AB - A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.

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Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

Abstract

All Science Journal Classification (ASJC) codes

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