Abstract
A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.
Original language | English |
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Pages (from-to) | 5880-5882 |
Number of pages | 3 |
Journal | Chemical Communications |
Issue number | 39 |
DOIs | |
Publication status | Published - Oct 12 2009 |
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All Science Journal Classification (ASJC) codes
- Catalysis
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Chemistry(all)
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry
Cite this
Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe. / Uchinomiya, Shohei; Nonaka, Hiroshi; Fujishima, Sho Hei; Tsukiji, Shinya; Ojida, Akio; Hamachi, Itaru.
In: Chemical Communications, No. 39, 12.10.2009, p. 5880-5882.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe
AU - Uchinomiya, Shohei
AU - Nonaka, Hiroshi
AU - Fujishima, Sho Hei
AU - Tsukiji, Shinya
AU - Ojida, Akio
AU - Hamachi, Itaru
PY - 2009/10/12
Y1 - 2009/10/12
N2 - A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.
AB - A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.
UR - http://www.scopus.com/inward/record.url?scp=70349682638&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349682638&partnerID=8YFLogxK
U2 - 10.1039/b912025d
DO - 10.1039/b912025d
M3 - Article
C2 - 19787128
AN - SCOPUS:70349682638
SP - 5880
EP - 5882
JO - Chemical Communications
JF - Chemical Communications
SN - 1359-7345
IS - 39
ER -