Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe

Sho Hei Uchinomiya, Hiroshi Nonaka, Sho Hei Fujishima, Shinya Tsukiji, Akio Ojida, Itaru Hamachi

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect.

Original languageEnglish
Pages (from-to)5880-5882
Number of pages3
JournalChemical Communications
Issue number39
DOIs
Publication statusPublished - Jan 1 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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